Construction Of Vectors For The Overexpression Of Recombinant Human Growth Hormone In Bacillus Megaterium
Bagi mengekspreskan gen hGH di dalam Bacillus megaterium, 3 konstrak telah direka. Konstrak-konstrak ini dinamakan sebagai Construct 1 (M7hGH), Construct 2 (R2L4sphGH) dan Construct 3 (spBsubhGH). Construct 1 direka dengan memasukkan gen pengawalatur (kodon penamat, tapak perlekatan ribosom, juj...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://eprints.usm.my/41298/1/Norhashima_Abd._Rashid24.pdf |
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| Summary: | Bagi mengekspreskan gen hGH di dalam Bacillus megaterium, 3 konstrak
telah direka. Konstrak-konstrak ini dinamakan sebagai Construct 1 (M7hGH),
Construct 2 (R2L4sphGH) dan Construct 3 (spBsubhGH). Construct 1 direka
dengan memasukkan gen pengawalatur (kodon penamat, tapak perlekatan ribosom,
jujukan TAACA dan kodon pemula) yang akan diekspreskan di dalam sel. Manakala
Construct 2 telah dicipta untuk diekpreskan di luar sel yang mana mengandungi
isyarat peptida daripada Bacillus brevis dan gen.
In order to express the hGH gene in Bacillus megaterium, 3 constructs were
designed. The constructs were named as Construct 1 (M7hGH), Construct 2
(R2L4sphGH) and Construct 3 (spBsubhGH). Construct 1 was designed with
regulatory features [stop codon, ribosome binding site (RBS), TAACA sequence and
start codon] meant for intracellular expression. Construct 2, was created to express
human growth hormone (hGH) extracellularly by using the signal peptide from
Bacillus brevis. |
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