Construction Of Vectors For The Overexpression Of Recombinant Human Growth Hormone In Bacillus Megaterium
Bagi mengekspreskan gen hGH di dalam Bacillus megaterium, 3 konstrak telah direka. Konstrak-konstrak ini dinamakan sebagai Construct 1 (M7hGH), Construct 2 (R2L4sphGH) dan Construct 3 (spBsubhGH). Construct 1 direka dengan memasukkan gen pengawalatur (kodon penamat, tapak perlekatan ribosom, juj...
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2009
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my-usm-ep.412982019-04-12T05:27:01Z Construction Of Vectors For The Overexpression Of Recombinant Human Growth Hormone In Bacillus Megaterium 2009-01 Rashid, Norhashima Abd. QH1 Natural history (General - Including nature conservation, geographical distribution) Bagi mengekspreskan gen hGH di dalam Bacillus megaterium, 3 konstrak telah direka. Konstrak-konstrak ini dinamakan sebagai Construct 1 (M7hGH), Construct 2 (R2L4sphGH) dan Construct 3 (spBsubhGH). Construct 1 direka dengan memasukkan gen pengawalatur (kodon penamat, tapak perlekatan ribosom, jujukan TAACA dan kodon pemula) yang akan diekspreskan di dalam sel. Manakala Construct 2 telah dicipta untuk diekpreskan di luar sel yang mana mengandungi isyarat peptida daripada Bacillus brevis dan gen. In order to express the hGH gene in Bacillus megaterium, 3 constructs were designed. The constructs were named as Construct 1 (M7hGH), Construct 2 (R2L4sphGH) and Construct 3 (spBsubhGH). Construct 1 was designed with regulatory features [stop codon, ribosome binding site (RBS), TAACA sequence and start codon] meant for intracellular expression. Construct 2, was created to express human growth hormone (hGH) extracellularly by using the signal peptide from Bacillus brevis. 2009-01 Thesis http://eprints.usm.my/41298/ http://eprints.usm.my/41298/1/Norhashima_Abd._Rashid24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
| spellingShingle |
QH1 Natural history (General - Including nature conservation geographical distribution) Rashid, Norhashima Abd. Construction Of Vectors For The Overexpression Of Recombinant Human Growth Hormone In Bacillus Megaterium |
| description |
Bagi mengekspreskan gen hGH di dalam Bacillus megaterium, 3 konstrak
telah direka. Konstrak-konstrak ini dinamakan sebagai Construct 1 (M7hGH),
Construct 2 (R2L4sphGH) dan Construct 3 (spBsubhGH). Construct 1 direka
dengan memasukkan gen pengawalatur (kodon penamat, tapak perlekatan ribosom,
jujukan TAACA dan kodon pemula) yang akan diekspreskan di dalam sel. Manakala
Construct 2 telah dicipta untuk diekpreskan di luar sel yang mana mengandungi
isyarat peptida daripada Bacillus brevis dan gen.
In order to express the hGH gene in Bacillus megaterium, 3 constructs were
designed. The constructs were named as Construct 1 (M7hGH), Construct 2
(R2L4sphGH) and Construct 3 (spBsubhGH). Construct 1 was designed with
regulatory features [stop codon, ribosome binding site (RBS), TAACA sequence and
start codon] meant for intracellular expression. Construct 2, was created to express
human growth hormone (hGH) extracellularly by using the signal peptide from
Bacillus brevis. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Rashid, Norhashima Abd. |
| author_facet |
Rashid, Norhashima Abd. |
| author_sort |
Rashid, Norhashima Abd. |
| title |
Construction Of Vectors For The
Overexpression Of Recombinant Human
Growth Hormone In Bacillus Megaterium
|
| title_short |
Construction Of Vectors For The
Overexpression Of Recombinant Human
Growth Hormone In Bacillus Megaterium
|
| title_full |
Construction Of Vectors For The
Overexpression Of Recombinant Human
Growth Hormone In Bacillus Megaterium
|
| title_fullStr |
Construction Of Vectors For The
Overexpression Of Recombinant Human
Growth Hormone In Bacillus Megaterium
|
| title_full_unstemmed |
Construction Of Vectors For The
Overexpression Of Recombinant Human
Growth Hormone In Bacillus Megaterium
|
| title_sort |
construction of vectors for the
overexpression of recombinant human
growth hormone in bacillus megaterium |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat |
| publishDate |
2009 |
| url |
http://eprints.usm.my/41298/1/Norhashima_Abd._Rashid24.pdf |
| _version_ |
1747820904991162368 |