Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice

IL-18 is a potent proinflammatory cytokine that plays multiple roles in immune responses and inflammatory activities in many disease conditions, but its involvement in the underlying pathogenesis of malaria has not been fully elucidated. In this study, the role and involvement of IL-18 during mal...

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Main Author:	Hasballah, Kartini
Format:	Thesis
Language:	English
Published:	2010
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/41639/1/KARTINI_HASBALLAH_HJ.pdf
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id	my-usm-ep.41639
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spelling	my-usm-ep.416392019-04-12T05:26:47Z Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice 2010-10 Hasballah, Kartini RS1-441 Pharmacy and materia medica IL-18 is a potent proinflammatory cytokine that plays multiple roles in immune responses and inflammatory activities in many disease conditions, but its involvement in the underlying pathogenesis of malaria has not been fully elucidated. In this study, the role and involvement of IL-18 during malaria infection was investigated and the impact of its pathway modulation on the course of the infection and the major cytokines released during the infection was preliminary evaluated. Plasmodium berghei ANKA infection in ICR mice were used as malaria model throughout the study. The animals were inoculated intravenously with 2 x 107 parasitized red blood cells (PRBCs) obtained from a donor mouse infected with the parasite. The control animals received an equivalent volume and dilution (0.2 mL, i.v.) of normal mouse RBC. Results demonstrated that the malarial mice showed sick behavioral changes on day 4 after inoculation when the levels of parasitaemia were ≥ 60% and then continued to increase until circulating parasitaemia reached around 80%. The infected mice succumbed to hyperparasitaemia 5-6 days after infection. ICR mice also showed significant decrease in body temperature and body weight during the peak parasitaemia. IL-18 concentrations in the plasma determined by means of ELISA, showed significant elevation throughout the infection and a positive correlation with parasitaemia development. 2010-10 Thesis http://eprints.usm.my/41639/ http://eprints.usm.my/41639/1/KARTINI_HASBALLAH_HJ.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Hasballah, Kartini Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice
description	IL-18 is a potent proinflammatory cytokine that plays multiple roles in immune responses and inflammatory activities in many disease conditions, but its involvement in the underlying pathogenesis of malaria has not been fully elucidated. In this study, the role and involvement of IL-18 during malaria infection was investigated and the impact of its pathway modulation on the course of the infection and the major cytokines released during the infection was preliminary evaluated. Plasmodium berghei ANKA infection in ICR mice were used as malaria model throughout the study. The animals were inoculated intravenously with 2 x 107 parasitized red blood cells (PRBCs) obtained from a donor mouse infected with the parasite. The control animals received an equivalent volume and dilution (0.2 mL, i.v.) of normal mouse RBC. Results demonstrated that the malarial mice showed sick behavioral changes on day 4 after inoculation when the levels of parasitaemia were ≥ 60% and then continued to increase until circulating parasitaemia reached around 80%. The infected mice succumbed to hyperparasitaemia 5-6 days after infection. ICR mice also showed significant decrease in body temperature and body weight during the peak parasitaemia. IL-18 concentrations in the plasma determined by means of ELISA, showed significant elevation throughout the infection and a positive correlation with parasitaemia development.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Hasballah, Kartini
author_facet	Hasballah, Kartini
author_sort	Hasballah, Kartini
title	Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice
title_short	Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice
title_full	Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice
title_fullStr	Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice
title_full_unstemmed	Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice
title_sort	investigating interleukin-18 involvement and its modulatory effects on major cytokines release during malaria infection in mice
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2010
url	http://eprints.usm.my/41639/1/KARTINI_HASBALLAH_HJ.pdf
_version_	1747820946570346496

Investigating Interleukin-18 Involvement And Its Modulatory Effects On Major Cytokines Release During Malaria Infection In Mice

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