Molecular Characterization Of Fusarium Oxysporum F. Sp. Cubense (Foc) In Malaysia
Sebanyak 59 pencilan Fusarium oxysporum f. sp. cubense (FOC) dianalisis menggunakan ERIC-PCR, RAMS dan RFLP-IGS. Ketiga-tiga teknik molekul ini menunjukkan variasi intraspesifik di mana pencilan-pencilan FOC menghasilkan 34 corak jalur ERIC, 33 haplotaip IGS dan 13 – 26 corak jalur mikrosatelit....
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| Format: | Thesis |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://eprints.usm.my/41815/1/LEONG_SAU_KUEEN24.pdf |
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| Summary: | Sebanyak 59 pencilan Fusarium oxysporum f. sp. cubense (FOC) dianalisis
menggunakan ERIC-PCR, RAMS dan RFLP-IGS. Ketiga-tiga teknik molekul ini
menunjukkan variasi intraspesifik di mana pencilan-pencilan FOC menghasilkan 34
corak jalur ERIC, 33 haplotaip IGS dan 13 – 26 corak jalur mikrosatelit. Corak jalur
yang dihasilkan melalui setiap teknik adalah sangat bervariasi, namun terdapat juga
persamaan corak jalur ditunjukkan oleh pencilan-pencilan FOC.
A total of 59 Fusarium oxysporum f. sp. cubense (FOC) isolates were analysed
using ERIC-PCR, RAMS and RFLP-IGS. The three molecular techniques showed
intraspecific variation among the FOC isolates, whereby 34 ERIC banding patterns,
33 IGS haplotypes and 13 – 26 microsatellite banding patterns were generated. The
banding patterns generated using each technique were highly variable, but showed
similarities among the FOC isolates. |
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