Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei
Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this stu...
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2010
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my-usm-ep.421672019-04-12T05:26:57Z Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei 2010-03 Shakrin, Nik Noorul Shakira Mohamed R735-854 Medical education. Medical schools. Research Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this study, thermostabilised Polymerase Chain Reaction (PCR) assays were developed for the detection of ompA genes in Shigella dysenteriae, Shigella flexneri and Shigella sonnei. The tests contained specific primers for the detection of S. dysenteriae, S. flexneri and S. sonnei respectively, freezed-dried or thermostabilised PCR reagents with Internal Control (IC) and gel loading dye. 2010-03 Thesis http://eprints.usm.my/42167/ http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
R735-854 Medical education Medical schools Research |
| spellingShingle |
R735-854 Medical education Medical schools Research Shakrin, Nik Noorul Shakira Mohamed Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| description |
Shigellosis or bacillary dysentery caused by Shigella spp. remains as significant health problem worldwide. Current detection and identification methods of the pathogens from stool specimens by culture method is relatively insensitive, generally time consuming (~ 2-5 days) and laborious. In this study, thermostabilised Polymerase Chain Reaction (PCR) assays were developed for the detection of ompA genes in Shigella dysenteriae, Shigella flexneri and Shigella sonnei. The tests contained specific primers for the detection of S. dysenteriae, S. flexneri and S. sonnei respectively, freezed-dried or thermostabilised PCR reagents with Internal Control (IC) and gel loading dye. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Shakrin, Nik Noorul Shakira Mohamed |
| author_facet |
Shakrin, Nik Noorul Shakira Mohamed |
| author_sort |
Shakrin, Nik Noorul Shakira Mohamed |
| title |
Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_short |
Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_full |
Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_fullStr |
Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_full_unstemmed |
Development Of DNA-Based Diagnostic Tests For The Detection Of Shigella Dysenteriae, Shigella Flexneri And Shigella Sonnei |
| title_sort |
development of dna-based diagnostic tests for the detection of shigella dysenteriae, shigella flexneri and shigella sonnei |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Institut Penyelidikan Perubatan Molekul |
| publishDate |
2010 |
| url |
http://eprints.usm.my/42167/1/NIK_NOORUL_SHAKIRA.pdf |
| _version_ |
1747821032997126144 |