Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2
The incorporation of a newly identified 3-hydroxy-4-methylvalerate (3H4MV) monomer into the backbone of polyhydroxyalkanoate (PHA) is thought to improve the properties of the resulting polymer. In this study, a transformant of C. necator PHB¯ 4 harboring the PHA synthase gene of Chromobacterium sp....
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2011
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my-usm-ep.422742019-04-12T05:26:37Z Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2 2011-05 Ooi , Tin Fong QH1 Natural history (General - Including nature conservation, geographical distribution) The incorporation of a newly identified 3-hydroxy-4-methylvalerate (3H4MV) monomer into the backbone of polyhydroxyalkanoate (PHA) is thought to improve the properties of the resulting polymer. In this study, a transformant of C. necator PHB¯ 4 harboring the PHA synthase gene of Chromobacterium sp. USM2 [C. necator PHB¯4 (phaCCs)] was evaluated for its ability to synthesize novel short-chain and medium-chain-length (SCL-MCL) PHA containing 3H4MV. Fructose or crude palm kernel oil (CPKO) was used as main carbon source together with isocaproic acid (4MV) as precursor to produce the excellent SCL-MCL PHA. 4MV concentration and its feeding time had been identified as important parameters affecting PHA content and monomer composition of the resulting polymer. 2011-05 Thesis http://eprints.usm.my/42274/ http://eprints.usm.my/42274/1/OOI_TIN_FONG.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
| spellingShingle |
QH1 Natural history (General - Including nature conservation geographical distribution) Ooi , Tin Fong Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2 |
| description |
The incorporation of a newly identified 3-hydroxy-4-methylvalerate (3H4MV) monomer into the backbone of polyhydroxyalkanoate (PHA) is thought to improve
the properties of the resulting polymer. In this study, a transformant of C. necator PHB¯ 4 harboring the PHA synthase gene of Chromobacterium sp. USM2 [C. necator PHB¯4 (phaCCs)] was evaluated for its ability to synthesize novel short-chain and medium-chain-length (SCL-MCL) PHA containing 3H4MV. Fructose or crude palm kernel oil (CPKO) was used as main carbon source together with isocaproic acid (4MV) as precursor to produce the excellent SCL-MCL PHA. 4MV concentration and its feeding time had been identified as important parameters
affecting PHA content and monomer composition of the resulting polymer. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Ooi , Tin Fong |
| author_facet |
Ooi , Tin Fong |
| author_sort |
Ooi , Tin Fong |
| title |
Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2 |
| title_short |
Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2 |
| title_full |
Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2 |
| title_fullStr |
Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2 |
| title_full_unstemmed |
Biosynthesis And Characterization Of Novel Polyhydroxyalkanoate By Cupriavidus Necator PHB-4 Transformant Harboring PHA Synthase Gene Of Chromobacterium SP. USM2 |
| title_sort |
biosynthesis and characterization of novel polyhydroxyalkanoate by cupriavidus necator phb-4 transformant harboring pha synthase gene of chromobacterium sp. usm2 |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat |
| publishDate |
2011 |
| url |
http://eprints.usm.my/42274/1/OOI_TIN_FONG.pdf |
| _version_ |
1747821048855789568 |