The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28
The vitrification and encapsulation-dehydration methods of cryopreservation were applied on protocorm-like bodies (PLBs) of the orchid hybrid Dendrobium sonia-28, with survival assessments conducted through growth observations, visualand spectrophotometric 2,3,5-triphenyltetrazolium chloride (TTC) a...
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2012
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my-usm-ep.423052019-04-12T05:26:23Z The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28 2012-02 Poobathy, Ranjetta QH1 Natural history (General - Including nature conservation, geographical distribution) The vitrification and encapsulation-dehydration methods of cryopreservation were applied on protocorm-like bodies (PLBs) of the orchid hybrid Dendrobium sonia-28, with survival assessments conducted through growth observations, visualand spectrophotometric 2,3,5-triphenyltetrazolium chloride (TTC) assays. The best survival (16.0% regeneration) for vitrification was obtained when 3-4mm PLBs were precultured in semi-solid half-strength MS medium supplemented with 0.4M sucrose, loaded in a loading solution for 20 minutes, dehydrated for 50 minutes at 0°C in plant vitrification solution 2 (PVS2), cryopreserved in liquid nitrogen (LN) for 24 hours, thawed in a 40±2°C water bath for 90 seconds, unloaded in 1.2M sucrose for 20 minutes, and regenerated in semi-solid half-strength MS medium containing 2g.L-1 charcoal, with all media supplemented with 0.6mM ascorbic acid. 2012-02 Thesis http://eprints.usm.my/42305/ http://eprints.usm.my/42305/1/RANJETTA_POOBATHY.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
| spellingShingle |
QH1 Natural history (General - Including nature conservation geographical distribution) Poobathy, Ranjetta The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28 |
| description |
The vitrification and encapsulation-dehydration methods of cryopreservation were applied on protocorm-like bodies (PLBs) of the orchid hybrid Dendrobium sonia-28, with survival assessments conducted through growth observations, visualand spectrophotometric 2,3,5-triphenyltetrazolium chloride (TTC) assays. The best
survival (16.0% regeneration) for vitrification was obtained when 3-4mm PLBs were precultured in semi-solid half-strength MS medium supplemented with 0.4M
sucrose, loaded in a loading solution for 20 minutes, dehydrated for 50 minutes at 0°C in plant vitrification solution 2 (PVS2), cryopreserved in liquid nitrogen (LN)
for 24 hours, thawed in a 40±2°C water bath for 90 seconds, unloaded in 1.2M sucrose for 20 minutes, and regenerated in semi-solid half-strength MS medium
containing 2g.L-1 charcoal, with all media supplemented with 0.6mM ascorbic acid. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Poobathy, Ranjetta |
| author_facet |
Poobathy, Ranjetta |
| author_sort |
Poobathy, Ranjetta |
| title |
The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28 |
| title_short |
The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28 |
| title_full |
The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28 |
| title_fullStr |
The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28 |
| title_full_unstemmed |
The Development Of Encapsulation-Dehydration And Vitrification Protocols For Protocorm-Like Bodies (PLBs) Of Dendrobium Sonia-28 |
| title_sort |
development of encapsulation-dehydration and vitrification protocols for protocorm-like bodies (plbs) of dendrobium sonia-28 |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat |
| publishDate |
2012 |
| url |
http://eprints.usm.my/42305/1/RANJETTA_POOBATHY.pdf |
| _version_ |
1747821056270270464 |