Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development

Cholera is a lethal diarrheal disease caused by toxigenic V. cholerae of O1 and O139 serogroups. Early diagnosis and prompt treatments of the disease are needed to prevent death in these patients. Even though the spreading of the disease could be controlled with proper sanitation, the best way to...

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Main Author:	Tan , Gim Cheong
Format:	Thesis
Language:	English
Published:	2010
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/42533/1/Tan_Gim_Cheong24.pdf
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id	my-usm-ep.42533
record_format	uketd_dc
spelling	my-usm-ep.425332019-04-12T05:26:59Z Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development 2010 Tan , Gim Cheong R5-920 Medicine (General) Cholera is a lethal diarrheal disease caused by toxigenic V. cholerae of O1 and O139 serogroups. Early diagnosis and prompt treatments of the disease are needed to prevent death in these patients. Even though the spreading of the disease could be controlled with proper sanitation, the best way to prevent cholera is through vaccination. There are several cholera vaccines available for O1 serogroup V. cholerae but they do not cross-protect against O139 serogroup. The bivalent O1/O139 cholera vaccine also did not show satisfactory protective immune response against O139 serogroup in clinical trials. Hence, the objective of this study is to construct an attenuated live cholera vaccine against V. cholerae O139 serogroup. In the present study, we focused on mutational analysis of MARTX toxin and cholera toxin of V. cholerae O139 serogroup. Constructed rtxC (VCUSM9P) and rtxA/C (VCUSM10P) mutants of V. cholerae O139 serogroup showed depleted in cytotoxic properties. 2010 Thesis http://eprints.usm.my/42533/ http://eprints.usm.my/42533/1/Tan_Gim_Cheong24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Tan , Gim Cheong Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development
description	Cholera is a lethal diarrheal disease caused by toxigenic V. cholerae of O1 and O139 serogroups. Early diagnosis and prompt treatments of the disease are needed to prevent death in these patients. Even though the spreading of the disease could be controlled with proper sanitation, the best way to prevent cholera is through vaccination. There are several cholera vaccines available for O1 serogroup V. cholerae but they do not cross-protect against O139 serogroup. The bivalent O1/O139 cholera vaccine also did not show satisfactory protective immune response against O139 serogroup in clinical trials. Hence, the objective of this study is to construct an attenuated live cholera vaccine against V. cholerae O139 serogroup. In the present study, we focused on mutational analysis of MARTX toxin and cholera toxin of V. cholerae O139 serogroup. Constructed rtxC (VCUSM9P) and rtxA/C (VCUSM10P) mutants of V. cholerae O139 serogroup showed depleted in cytotoxic properties.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Tan , Gim Cheong
author_facet	Tan , Gim Cheong
author_sort	Tan , Gim Cheong
title	Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development
title_short	Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development
title_full	Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development
title_fullStr	Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development
title_full_unstemmed	Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development
title_sort	understanding the molecular enteropathogenesis of martx toxin genes and cholera toxin gene mutations of vibrio cholerae o139 serogroup towards cholera vaccine development
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2010
url	http://eprints.usm.my/42533/1/Tan_Gim_Cheong24.pdf
_version_	1747821080957943808

Understanding The Molecular Enteropathogenesis Of Martx Toxin Genes And Cholera Toxin Gene Mutations Of Vibrio Cholerae O139 Serogroup Towards Cholera Vaccine Development

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