Identification Of Salmonella Enterica Subspecies Enterica Serovar Typhi-Specific Genes For The Development Of Dna-Based And Antibody-Based Diagnostics For Typhoid Fever
Typhoid fever is caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi). It is an acute systemic disease which remains a major public health burden worldwide. A lack of specific and sensitive diagnostic markers for detection of S. Typhi at single-gene target resolution prevents e...
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2018
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my-usm-ep.429042019-04-12T05:24:52Z Identification Of Salmonella Enterica Subspecies Enterica Serovar Typhi-Specific Genes For The Development Of Dna-Based And Antibody-Based Diagnostics For Typhoid Fever 2018-02 Goay , Yuan Xin R735-854 Medical education. Medical schools. Research Typhoid fever is caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi). It is an acute systemic disease which remains a major public health burden worldwide. A lack of specific and sensitive diagnostic markers for detection of S. Typhi at single-gene target resolution prevents effective diagnosis and therefore efficient control of the disease. In the first phase of this study, genome level comparison of S. Typhi with other enteric pathogens was performed using BLASTn. Six genes, i.e. STY0201, STY0307, STY0322, STY0326, STY2020 and STY2021 were found to be specific and sensitive in silico. Six individual single-gene target PCR assays with the incorporation of an internal amplification control (IAC) were developed and optimised using Taguchi method. 2018-02 Thesis http://eprints.usm.my/42904/ http://eprints.usm.my/42904/1/GOAY_YUAN_XIN.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
R735-854 Medical education Medical schools Research |
| spellingShingle |
R735-854 Medical education Medical schools Research Goay , Yuan Xin Identification Of Salmonella Enterica Subspecies Enterica Serovar Typhi-Specific Genes For The Development Of Dna-Based And Antibody-Based Diagnostics For Typhoid Fever |
| description |
Typhoid fever is caused by Salmonella enterica subspecies enterica serovar Typhi (S. Typhi). It is an acute systemic disease which remains a major public health burden worldwide. A lack of specific and sensitive diagnostic markers for detection of S. Typhi at single-gene target resolution prevents effective diagnosis and therefore efficient control of the disease. In the first phase of this study, genome level comparison of S. Typhi with other enteric pathogens was performed using BLASTn. Six genes, i.e. STY0201, STY0307, STY0322, STY0326, STY2020 and STY2021 were found to be specific and sensitive in silico. Six individual single-gene target PCR assays with the incorporation of an internal amplification control (IAC) were developed and optimised using Taguchi method. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Goay , Yuan Xin |
| author_facet |
Goay , Yuan Xin |
| author_sort |
Goay , Yuan Xin |
| title |
Identification Of Salmonella Enterica Subspecies Enterica Serovar Typhi-Specific Genes For The Development Of
Dna-Based And Antibody-Based Diagnostics For Typhoid Fever
|
| title_short |
Identification Of Salmonella Enterica Subspecies Enterica Serovar Typhi-Specific Genes For The Development Of
Dna-Based And Antibody-Based Diagnostics For Typhoid Fever
|
| title_full |
Identification Of Salmonella Enterica Subspecies Enterica Serovar Typhi-Specific Genes For The Development Of
Dna-Based And Antibody-Based Diagnostics For Typhoid Fever
|
| title_fullStr |
Identification Of Salmonella Enterica Subspecies Enterica Serovar Typhi-Specific Genes For The Development Of
Dna-Based And Antibody-Based Diagnostics For Typhoid Fever
|
| title_full_unstemmed |
Identification Of Salmonella Enterica Subspecies Enterica Serovar Typhi-Specific Genes For The Development Of
Dna-Based And Antibody-Based Diagnostics For Typhoid Fever
|
| title_sort |
identification of salmonella enterica subspecies enterica serovar typhi-specific genes for the development of
dna-based and antibody-based diagnostics for typhoid fever |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Institut Penyelidikan Perubatan Molekul |
| publishDate |
2018 |
| url |
http://eprints.usm.my/42904/1/GOAY_YUAN_XIN.pdf |
| _version_ |
1747821124222189568 |