The Role Of Bcsa Gene In Cellulose Biosynthesis Of Burkholderia Pseudomallei
Two copies of cellulose synthase gene were identified in the genome database of B. pseudomallei K96243. Both genes are located on the chromosome II and labelled as open reading frame (ORF) BPSS0735 and BPSS1577, respectively. Sequence comparisons revealed that the two genes share low similari...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://eprints.usm.my/42911/1/Chai_Muh_Chyi24.pdf |
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| Summary: | Two copies of cellulose synthase gene were identified in the genome database of B.
pseudomallei K96243. Both genes are located on the chromosome II and labelled as
open reading frame (ORF) BPSS0735 and BPSS1577, respectively. Sequence
comparisons revealed that the two genes share low similarity and identity of 35% and
24%, respectively. This suggested that the two cellulose synthase genes are of different
origins. In this study, B. pseudomallei was investigated for the biosynthesis of cellulose
and experimental evidences of the presence of cellulose were shown. Hence, a study was
conducted to characterize the role of BPSS1577 (also annotated as bcsA) in the cellulose
biosynthesis of B. pseudomallei. A bcsA deleted mutant was generated to determine
whether bcsA was involved in biofilm formation, adherence to abiotic surfaces and
desiccation survival potential in this bacterium. |
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