Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4

Detection of hydrocarbon-degrading enzymes activities in microorganisms capable of utilizing crude oil as a carbon source were done using potential oil-degraders isolated from soil and water samples collected from water and oil-contaminated areas in Penang. Seven potential isolates categorized...

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Main Author:	Chee , Mei Sing
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/42914/1/CHEE_MEI_SING24.pdf
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id	my-usm-ep.42914
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spelling	my-usm-ep.429142019-04-12T05:26:55Z Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4 2010-03 Chee , Mei Sing QH1 Natural history (General - Including nature conservation, geographical distribution) Detection of hydrocarbon-degrading enzymes activities in microorganisms capable of utilizing crude oil as a carbon source were done using potential oil-degraders isolated from soil and water samples collected from water and oil-contaminated areas in Penang. Seven potential isolates categorized as Group I bacteria were selected based on the criteria that they displayed good growth on solid crude oil medium using 6 different types of crude oil which were named as. Tapis, Khefji Dubai, Bunga Kekwa, Angsi, Dulang, and Penara. These crude oil media were prepared without the addition of surfactant. Out of seven isolates, oil-degrading bacterium designated as FETL C4 displayed good growth on different types of crude oil media prepared. Biochemical test results identified this isolate as Acinetobacter sp. Further confirmation was done using 16S rRNA gene analysis and Remel Rapid Identification Kit. Aldehyde dehydrogenase (ALDH) activity was detected during the cultivation of this isolate when grown in minimal salt medium containing 1% (w/v) Tapis crude as a carbon source. Optimizations on the production of this enzyme were done by manipulating physical and chemical parameters of the growth and enzyme production. The optimization process were carried out in a flask system (250 mL) and then further scaled up via fermenter system (5 L). Cells were harvested from the growth medium when maximum points were reached for both enzyme activity and degradation of the crude oil. The optimal conditions when aldehyde dehydrogenase activity detected at the highest rate were obtained at 0.2 giL (NH4)2S04, 0.4 giL KH2P04, 0.8 giL Na2HP04, 1.0 giL Tapis crude, 0.2 giL Tween 80 as surfactant, cultivation temperature at 37°C, initial medium pH 8.0, with addition of 5% inoculum (1 x 106 cells/mL) and agitated at 600 rpm. Aeration rate was set at 2L per minute to enhance the growth of the isolate. Biomass production and degradation rate of the crude oil were 2.0 giL and 70%, respectively. Zymogram analysis of aldehyde dehydrogenase on different aldehyde substrates revealed that only one activity band that involved in the metabolism of various aldehyde substrates. Quantitative analysis of this enzyme on different aldehyde substrates showed a higher preference towards aldehyde with carbon 12. 2010-03 Thesis http://eprints.usm.my/42914/ http://eprints.usm.my/42914/1/CHEE_MEI_SING24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Chee , Mei Sing Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4
description	Detection of hydrocarbon-degrading enzymes activities in microorganisms capable of utilizing crude oil as a carbon source were done using potential oil-degraders isolated from soil and water samples collected from water and oil-contaminated areas in Penang. Seven potential isolates categorized as Group I bacteria were selected based on the criteria that they displayed good growth on solid crude oil medium using 6 different types of crude oil which were named as. Tapis, Khefji Dubai, Bunga Kekwa, Angsi, Dulang, and Penara. These crude oil media were prepared without the addition of surfactant. Out of seven isolates, oil-degrading bacterium designated as FETL C4 displayed good growth on different types of crude oil media prepared. Biochemical test results identified this isolate as Acinetobacter sp. Further confirmation was done using 16S rRNA gene analysis and Remel Rapid Identification Kit. Aldehyde dehydrogenase (ALDH) activity was detected during the cultivation of this isolate when grown in minimal salt medium containing 1% (w/v) Tapis crude as a carbon source. Optimizations on the production of this enzyme were done by manipulating physical and chemical parameters of the growth and enzyme production. The optimization process were carried out in a flask system (250 mL) and then further scaled up via fermenter system (5 L). Cells were harvested from the growth medium when maximum points were reached for both enzyme activity and degradation of the crude oil. The optimal conditions when aldehyde dehydrogenase activity detected at the highest rate were obtained at 0.2 giL (NH4)2S04, 0.4 giL KH2P04, 0.8 giL Na2HP04, 1.0 giL Tapis crude, 0.2 giL Tween 80 as surfactant, cultivation temperature at 37°C, initial medium pH 8.0, with addition of 5% inoculum (1 x 106 cells/mL) and agitated at 600 rpm. Aeration rate was set at 2L per minute to enhance the growth of the isolate. Biomass production and degradation rate of the crude oil were 2.0 giL and 70%, respectively. Zymogram analysis of aldehyde dehydrogenase on different aldehyde substrates revealed that only one activity band that involved in the metabolism of various aldehyde substrates. Quantitative analysis of this enzyme on different aldehyde substrates showed a higher preference towards aldehyde with carbon 12.
format	Thesis
qualification_level	Master's degree
author	Chee , Mei Sing
author_facet	Chee , Mei Sing
author_sort	Chee , Mei Sing
title	Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4
title_short	Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4
title_full	Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4
title_fullStr	Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4
title_full_unstemmed	Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4
title_sort	effect of crude aldehyde dehydrogenase on the degradation of crude oil by a local isolate, acinetobacter baumannii fetl c4
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2010
url	http://eprints.usm.my/42914/1/CHEE_MEI_SING24.pdf
_version_	1747821126670614528

Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4

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