Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti

Callus of Cyperus aromaticus was induced from the root explants using modified Murashige and Skoog (1962) medium supplemented with 5.5 mg/L NAA and 4.5 mg/L 2,4-D. Seven stable cell lines were selected from these root-derived calluses. Cell suspension cultures were then established from these selec...

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Main Author:	Lim , Pey Shan
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/42990/1/Lim_Pey_Shan24.pdf
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id	my-usm-ep.42990
record_format	uketd_dc
spelling	my-usm-ep.429902019-04-12T05:26:51Z Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti 2010-06 Lim , Pey Shan QH1 Natural history (General - Including nature conservation, geographical distribution) Callus of Cyperus aromaticus was induced from the root explants using modified Murashige and Skoog (1962) medium supplemented with 5.5 mg/L NAA and 4.5 mg/L 2,4-D. Seven stable cell lines were selected from these root-derived calluses. Cell suspension cultures were then established from these selected cell lines. Optimum cell biomass and high Juvenile Hormone III (JH III) content could be produced via single stage cell culture system using a modified culture medium. JH III of the tested samples was detected with high performance liquid chromatography (HPLC). The optimum culture duration for the production of high cell biomass and JH III content was 12 days. The optimized culture medium was formulated via step-wise modification of macronutrients portion of the basic MS medium and plant growth regulators. All the selected cell lines grew well in this optimum medium which consisted of MS supplemented with 271.1 mM NH4NO3 + 15.4 mM KNO3 + 4.3 mM CaCl2.2H2O + 1.49 mM MgSO4.4H2O + 1.35 mM KH2PO4 + 3% sucrose + 5.5 mg/L NAA + 4.5 mg/L 2,4-D (MSB) medium. 2010-06 Thesis http://eprints.usm.my/42990/ http://eprints.usm.my/42990/1/Lim_Pey_Shan24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Lim , Pey Shan Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti
description	Callus of Cyperus aromaticus was induced from the root explants using modified Murashige and Skoog (1962) medium supplemented with 5.5 mg/L NAA and 4.5 mg/L 2,4-D. Seven stable cell lines were selected from these root-derived calluses. Cell suspension cultures were then established from these selected cell lines. Optimum cell biomass and high Juvenile Hormone III (JH III) content could be produced via single stage cell culture system using a modified culture medium. JH III of the tested samples was detected with high performance liquid chromatography (HPLC). The optimum culture duration for the production of high cell biomass and JH III content was 12 days. The optimized culture medium was formulated via step-wise modification of macronutrients portion of the basic MS medium and plant growth regulators. All the selected cell lines grew well in this optimum medium which consisted of MS supplemented with 271.1 mM NH4NO3 + 15.4 mM KNO3 + 4.3 mM CaCl2.2H2O + 1.49 mM MgSO4.4H2O + 1.35 mM KH2PO4 + 3% sucrose + 5.5 mg/L NAA + 4.5 mg/L 2,4-D (MSB) medium.
format	Thesis
qualification_level	Master's degree
author	Lim , Pey Shan
author_facet	Lim , Pey Shan
author_sort	Lim , Pey Shan
title	Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti
title_short	Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti
title_full	Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti
title_fullStr	Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti
title_full_unstemmed	Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti
title_sort	production of juvenile hormone iii (jh iii) from cyperus aromaticus via cell suspension culture technique and its effect on mosquito larva, aedes aegypti
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2010
url	http://eprints.usm.my/42990/1/Lim_Pey_Shan24.pdf
_version_	1747821143280058368

Production Of Juvenile Hormone Iii (Jh Iii) From Cyperus Aromaticus Via Cell Suspension Culture Technique And Its Effect On Mosquito Larva, Aedes Aegypti

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