Association of mitochondrial DNA 10398 polymorphism with breast cancer and apoptosis in Malay population.

Polimorfisma 10398 mitokondria DNA telah didapati berkait dengan kanser payu dara dalam beberapa populasi. Dalam kajian ini, polimorfisma 10398 mitokondria DNA telah disaring pada 101 pesakit kanser payu dara wanita Melayu dan 90 wanita yang sihat sebagai kawalan dengan menggunakan kaedah penjujuka...

Full description

Saved in:
Bibliographic Details
Main Author: Shah, Tengku Puteri Nadiah Tengku Baharudin
Format: Thesis
Language:English
Published: 2011
Subjects:
Online Access:http://eprints.usm.my/43114/1/Pages_from_ASSOCIATION_OF_MITOCHONDRIAL_DNA.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-usm-ep.43114
record_format uketd_dc
spelling my-usm-ep.431142018-12-07T08:42:20Z Association of mitochondrial DNA 10398 polymorphism with breast cancer and apoptosis in Malay population. 2011-07 Shah, Tengku Puteri Nadiah Tengku Baharudin R5-130.5 General works Polimorfisma 10398 mitokondria DNA telah didapati berkait dengan kanser payu dara dalam beberapa populasi. Dalam kajian ini, polimorfisma 10398 mitokondria DNA telah disaring pada 101 pesakit kanser payu dara wanita Melayu dan 90 wanita yang sihat sebagai kawalan dengan menggunakan kaedah penjujukan mini. Hasil menunjukkan perbezaan statistik yang signifikan dengan nilai P, p = 0.007 (OR, 2.29; 95% CI, 2.252- 4.200) dengan jumlah varian G lebih tinggi (73%) daripada varian A (27%) dalam pesakit dan juga dalam kawalan (G = 54%, A = 46%). Tisu kanser payudara kemudian dianalisis menggunakan kaedah immunohistokimia bagi mengkaji perkaitan polimorfisma ini dalam mempengaruhi aras apoptosis sel. Tiada perbezaan signifikan didapati antara aras pengekspresan protein pro (Bax) dan anti (Bcl-2) apoptosis antara pesakit yang membawa varian A ( p = 0.48). Bagaimanapun, terdapat perkaitan yang signifikan antara proteinprotein ini dalam pesakit yang mempunyai varian G (p = 0.016). Hasil ini menunjukkan polimorfisma 10398 mtDNA mungkin berguna sebagai penanda risiko kanser payu dara dalam populasi ini. The mitochondrial DNA 10398 polymorphism has been observed to associate with breast cancer in several populations. In this study, mitochondrial DNA 10398 polymorphism was screened in 101 Malay female patients with invasive breast cancer and 90 age-matched healthy female controls using minisequencing method. The results showed a statistically significant difference with P value of p = 0.007 (OR, 2.29; 95% CI, 1.252-4.200) with the proportion of G variant was higher (73%) than A variant in patients (27%) as well as in controls (G = 54%, A = 46%). The breast cancer tissues were then analyzed using immunohistochemistry method to investigate the relation of this polymorphism in affecting apoptotic level in breast cancer. No significant difference was observed between the expression of pro (Bax) and anti-apoptotic (Bcl-2) proteins among patients carrying A variant (p = 0.48). However, significant difference was observed in patients with G variant (p = 0.016). These results indicate that mtDNA 10398 polymorphism may be useful as a breast cancer risk marker in this population. 2011-07 Thesis http://eprints.usm.my/43114/ http://eprints.usm.my/43114/1/Pages_from_ASSOCIATION_OF_MITOCHONDRIAL_DNA.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-130.5 General works
spellingShingle R5-130.5 General works
Shah, Tengku Puteri Nadiah Tengku Baharudin
Association of mitochondrial DNA 10398 polymorphism with breast cancer and apoptosis in Malay population.
description Polimorfisma 10398 mitokondria DNA telah didapati berkait dengan kanser payu dara dalam beberapa populasi. Dalam kajian ini, polimorfisma 10398 mitokondria DNA telah disaring pada 101 pesakit kanser payu dara wanita Melayu dan 90 wanita yang sihat sebagai kawalan dengan menggunakan kaedah penjujukan mini. Hasil menunjukkan perbezaan statistik yang signifikan dengan nilai P, p = 0.007 (OR, 2.29; 95% CI, 2.252- 4.200) dengan jumlah varian G lebih tinggi (73%) daripada varian A (27%) dalam pesakit dan juga dalam kawalan (G = 54%, A = 46%). Tisu kanser payudara kemudian dianalisis menggunakan kaedah immunohistokimia bagi mengkaji perkaitan polimorfisma ini dalam mempengaruhi aras apoptosis sel. Tiada perbezaan signifikan didapati antara aras pengekspresan protein pro (Bax) dan anti (Bcl-2) apoptosis antara pesakit yang membawa varian A ( p = 0.48). Bagaimanapun, terdapat perkaitan yang signifikan antara proteinprotein ini dalam pesakit yang mempunyai varian G (p = 0.016). Hasil ini menunjukkan polimorfisma 10398 mtDNA mungkin berguna sebagai penanda risiko kanser payu dara dalam populasi ini. The mitochondrial DNA 10398 polymorphism has been observed to associate with breast cancer in several populations. In this study, mitochondrial DNA 10398 polymorphism was screened in 101 Malay female patients with invasive breast cancer and 90 age-matched healthy female controls using minisequencing method. The results showed a statistically significant difference with P value of p = 0.007 (OR, 2.29; 95% CI, 1.252-4.200) with the proportion of G variant was higher (73%) than A variant in patients (27%) as well as in controls (G = 54%, A = 46%). The breast cancer tissues were then analyzed using immunohistochemistry method to investigate the relation of this polymorphism in affecting apoptotic level in breast cancer. No significant difference was observed between the expression of pro (Bax) and anti-apoptotic (Bcl-2) proteins among patients carrying A variant (p = 0.48). However, significant difference was observed in patients with G variant (p = 0.016). These results indicate that mtDNA 10398 polymorphism may be useful as a breast cancer risk marker in this population.
format Thesis
qualification_level Master's degree
author Shah, Tengku Puteri Nadiah Tengku Baharudin
author_facet Shah, Tengku Puteri Nadiah Tengku Baharudin
author_sort Shah, Tengku Puteri Nadiah Tengku Baharudin
title Association of mitochondrial DNA 10398 polymorphism with breast cancer and apoptosis in Malay population.
title_short Association of mitochondrial DNA 10398 polymorphism with breast cancer and apoptosis in Malay population.
title_full Association of mitochondrial DNA 10398 polymorphism with breast cancer and apoptosis in Malay population.
title_fullStr Association of mitochondrial DNA 10398 polymorphism with breast cancer and apoptosis in Malay population.
title_full_unstemmed Association of mitochondrial DNA 10398 polymorphism with breast cancer and apoptosis in Malay population.
title_sort association of mitochondrial dna 10398 polymorphism with breast cancer and apoptosis in malay population.
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kesihatan
publishDate 2011
url http://eprints.usm.my/43114/1/Pages_from_ASSOCIATION_OF_MITOCHONDRIAL_DNA.pdf
_version_ 1747821167481192448