The Molecular Mechanisms Of Rapamycininduced Autophagy And Apoptosis In T-47d Breast Carcinoma Cells
Autophagy is an evolutionarily conserved lysosomal degradation pathway that leads to degradation of proteins and entire organelles and subsequently plays a crucial role in the homeostatic process of recycling proteins and organelles. Autophagy is an important biological mechanism that enables cel...
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2013
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my-usm-ep.432192019-04-12T05:26:17Z The Molecular Mechanisms Of Rapamycininduced Autophagy And Apoptosis In T-47d Breast Carcinoma Cells 2013-04 Moad, Ahmed Ismail Hassan RK1-715 Dentistry Autophagy is an evolutionarily conserved lysosomal degradation pathway that leads to degradation of proteins and entire organelles and subsequently plays a crucial role in the homeostatic process of recycling proteins and organelles. Autophagy is an important biological mechanism that enables cell survival and to induce death of damage cells. There is increasing evidence that autophagy progresses to autophagic cell death when the process is over-stimulated. Functional relationships have been described between apoptosis (Type I cell death) and autophagic cell death (Type II cell death). However, the molecular relationships and the circumstances of which molecular pathways dictate the choice between autophagy and apoptosis are currently unknown. Autophagy is regulated by the mammalian target of rapamycin (mTOR) kinase pathway. The mTOR are known to inhibit the autophagy process and subsequently lead to tumor development. As a strategy, rapamycin, a known inhibitor of mTOR, was used as an autophagy inducer and 3-methyladenine (3MA) as a classical inhibitor of autophagy. In the present study, a systematic global gene expression was investigated in rapamycin-induced autophagy and the effects of rapamycin when autophagy process is inhibited. The findings have demonstrated that rapamycin was capable of inducing autophagy in T-47D breast carcinoma cells. However, when autophagy was inhibited by 3MA, rapamycin appeared to induce apoptosis in these breast cells. 2013-04 Thesis http://eprints.usm.my/43219/ http://eprints.usm.my/43219/1/Ahmed%20Ismail%20Hassan%20Moad24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju |
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Universiti Sains Malaysia |
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USM Institutional Repository |
| language |
English |
| topic |
RK1-715 Dentistry |
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RK1-715 Dentistry Moad, Ahmed Ismail Hassan The Molecular Mechanisms Of Rapamycininduced Autophagy And Apoptosis In T-47d Breast Carcinoma Cells |
| description |
Autophagy is an evolutionarily conserved lysosomal degradation pathway
that leads to degradation of proteins and entire organelles and subsequently plays a
crucial role in the homeostatic process of recycling proteins and organelles.
Autophagy is an important biological mechanism that enables cell survival and to
induce death of damage cells. There is increasing evidence that autophagy progresses
to autophagic cell death when the process is over-stimulated. Functional relationships
have been described between apoptosis (Type I cell death) and autophagic cell death
(Type II cell death). However, the molecular relationships and the circumstances of
which molecular pathways dictate the choice between autophagy and apoptosis are
currently unknown. Autophagy is regulated by the mammalian target of rapamycin
(mTOR) kinase pathway. The mTOR are known to inhibit the autophagy process and
subsequently lead to tumor development. As a strategy, rapamycin, a known
inhibitor of mTOR, was used as an autophagy inducer and 3-methyladenine (3MA)
as a classical inhibitor of autophagy. In the present study, a systematic global gene
expression was investigated in rapamycin-induced autophagy and the effects of
rapamycin when autophagy process is inhibited. The findings have demonstrated that
rapamycin was capable of inducing autophagy in T-47D breast carcinoma cells.
However, when autophagy was inhibited by 3MA, rapamycin appeared to induce
apoptosis in these breast cells. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Moad, Ahmed Ismail Hassan |
| author_facet |
Moad, Ahmed Ismail Hassan |
| author_sort |
Moad, Ahmed Ismail Hassan |
| title |
The Molecular Mechanisms Of Rapamycininduced
Autophagy And Apoptosis In T-47d Breast
Carcinoma Cells |
| title_short |
The Molecular Mechanisms Of Rapamycininduced
Autophagy And Apoptosis In T-47d Breast
Carcinoma Cells |
| title_full |
The Molecular Mechanisms Of Rapamycininduced
Autophagy And Apoptosis In T-47d Breast
Carcinoma Cells |
| title_fullStr |
The Molecular Mechanisms Of Rapamycininduced
Autophagy And Apoptosis In T-47d Breast
Carcinoma Cells |
| title_full_unstemmed |
The Molecular Mechanisms Of Rapamycininduced
Autophagy And Apoptosis In T-47d Breast
Carcinoma Cells |
| title_sort |
molecular mechanisms of rapamycininduced
autophagy and apoptosis in t-47d breast
carcinoma cells |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Institut Perubatan & Pergigian Termaju |
| publishDate |
2013 |
| url |
http://eprints.usm.my/43219/1/Ahmed%20Ismail%20Hassan%20Moad24.pdf |
| _version_ |
1747821180230828032 |