Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates

Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were desig...

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Main Author:	Murugaiah, Chandrika
Format:	Thesis
Language:	English
Published:	2011
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/43233/1/CHANDRIKA%20MURUGAIAH.pdf
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id	my-usm-ep.43233
record_format	uketd_dc
spelling	my-usm-ep.432332019-04-12T05:26:31Z Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates 2011-09 Murugaiah, Chandrika R5-920 Medicine (General) Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were designed. VCUSM21P is a prototrophic vaccine which encodes non-toxic cholera toxin A (ctxA) subunit immunogen and has accessory cholera enterotoxin (ace), zonula occludens toxin (zot) and repeats-in-toxin C/A (rtxC/A) mutations. On the other hand, VCUSM22P is ace, zot, ctxA, rtxC/A and haemagglutinin/protease (hap) mutant. Both mutants were found not to disassemble the actin of HEp2 cells. 2011-09 Thesis http://eprints.usm.my/43233/ http://eprints.usm.my/43233/1/CHANDRIKA%20MURUGAIAH.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Murugaiah, Chandrika Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
description	Cholera is a major health issue, affecting millions of lives annually. In light of the recurrent outbreaks of cholera, there is a pressing need for the development of vaccines that allow rapid mass vaccination. In this study, genetically modified vaccine candidates, VCUSM21P and VCUSM22P, were designed. VCUSM21P is a prototrophic vaccine which encodes non-toxic cholera toxin A (ctxA) subunit immunogen and has accessory cholera enterotoxin (ace), zonula occludens toxin (zot) and repeats-in-toxin C/A (rtxC/A) mutations. On the other hand, VCUSM22P is ace, zot, ctxA, rtxC/A and haemagglutinin/protease (hap) mutant. Both mutants were found not to disassemble the actin of HEp2 cells.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Murugaiah, Chandrika
author_facet	Murugaiah, Chandrika
author_sort	Murugaiah, Chandrika
title	Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_short	Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_full	Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_fullStr	Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_full_unstemmed	Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates
title_sort	construction and evaluation of multigene mutants of vibrio cholerae o139 as vaccine candidates
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2011
url	http://eprints.usm.my/43233/1/CHANDRIKA%20MURUGAIAH.pdf
_version_	1747821183177326592

Construction And Evaluation Of Multigene Mutants Of Vibrio Cholerae O139 As Vaccine Candidates

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