Recombinant Erythropoietin Extraction By Immunoaffinity Column And The Detection Of Isoforms Using Capillary Electrophoresis
Erythropoietin (EPO) is an endogenous glycoprotein hormone. It is synthesized in and secreted mostly by the kidneys. In the market, there are a number of pharmaceuticals of recombinant EPO (rEPO) for clinical use. With the advent of novel technologies in the pharmaceutical and therapeutic area...
Saved in:
Main Author: | |
---|---|
Format: | Thesis |
Language: | English |
Published: |
2013
|
Subjects: | |
Online Access: | http://eprints.usm.my/43468/1/Hend%20Sultan%20A.S.%20Al-Jaber24.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my-usm-ep.43468 |
---|---|
record_format |
uketd_dc |
spelling |
my-usm-ep.434682019-04-12T05:26:17Z Recombinant Erythropoietin Extraction By Immunoaffinity Column And The Detection Of Isoforms Using Capillary Electrophoresis 2013-04 Al-Jaber, Hend Sultan A.S. RS1-441 Pharmacy and materia medica Erythropoietin (EPO) is an endogenous glycoprotein hormone. It is synthesized in and secreted mostly by the kidneys. In the market, there are a number of pharmaceuticals of recombinant EPO (rEPO) for clinical use. With the advent of novel technologies in the pharmaceutical and therapeutic areas, recombinant EPO have been introduced into the market. Although recombinant EPO has been broadly used to treat various forms of anaemia, it has also become a very well known drug due to its misuse in endurance sports. Sport authorities have prohibited the use of rEPO since 1989. This work has used a number of techniques to purify natural EPO and the recombinant form from the urine. Two Immunoaffinity column techniques (IAC) were used to purify EPO i.e. HiTrap Affinity Columns and MAIIA EPO Purification Kit. Both columns were successfully able to trap EPO molecules. EPO recovery from the HiTrap columns was negligible < 1%, whereas the recovery of MAIIA affinity column was satisfactory. The work has also developed a novel characterization tool using Capillary Electrophoresis (CE) that can be used in pharmaceutical Quality Control (QC) and has been investigated for its ability to detect drug misuse in sport. Conventional CE tools generally use a label that attaches to the EPO for identification. 2013-04 Thesis http://eprints.usm.my/43468/ http://eprints.usm.my/43468/1/Hend%20Sultan%20A.S.%20Al-Jaber24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Penyelidikan Dadah dan Ubat-ubatan |
institution |
Universiti Sains Malaysia |
collection |
USM Institutional Repository |
language |
English |
topic |
RS1-441 Pharmacy and materia medica |
spellingShingle |
RS1-441 Pharmacy and materia medica Al-Jaber, Hend Sultan A.S. Recombinant Erythropoietin Extraction By Immunoaffinity Column And The Detection Of Isoforms Using Capillary Electrophoresis |
description |
Erythropoietin (EPO) is an endogenous glycoprotein hormone. It is
synthesized in and secreted mostly by the kidneys. In the market, there are a number of
pharmaceuticals of recombinant EPO (rEPO) for clinical use. With the advent of novel
technologies in the pharmaceutical and therapeutic areas, recombinant EPO have been
introduced into the market. Although recombinant EPO has been broadly used to treat
various forms of anaemia, it has also become a very well known drug due to its misuse
in endurance sports. Sport authorities have prohibited the use of rEPO since 1989. This
work has used a number of techniques to purify natural EPO and the recombinant form
from the urine. Two Immunoaffinity column techniques (IAC) were used to purify
EPO i.e. HiTrap Affinity Columns and MAIIA EPO Purification Kit. Both columns
were successfully able to trap EPO molecules. EPO recovery from the HiTrap columns
was negligible < 1%, whereas the recovery of MAIIA affinity column was satisfactory.
The work has also developed a novel characterization tool using Capillary
Electrophoresis (CE) that can be used in pharmaceutical Quality Control (QC) and has
been investigated for its ability to detect drug misuse in sport. Conventional CE tools
generally use a label that attaches to the EPO for identification. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Al-Jaber, Hend Sultan A.S. |
author_facet |
Al-Jaber, Hend Sultan A.S. |
author_sort |
Al-Jaber, Hend Sultan A.S. |
title |
Recombinant Erythropoietin
Extraction By Immunoaffinity Column
And The Detection Of Isoforms Using
Capillary Electrophoresis |
title_short |
Recombinant Erythropoietin
Extraction By Immunoaffinity Column
And The Detection Of Isoforms Using
Capillary Electrophoresis |
title_full |
Recombinant Erythropoietin
Extraction By Immunoaffinity Column
And The Detection Of Isoforms Using
Capillary Electrophoresis |
title_fullStr |
Recombinant Erythropoietin
Extraction By Immunoaffinity Column
And The Detection Of Isoforms Using
Capillary Electrophoresis |
title_full_unstemmed |
Recombinant Erythropoietin
Extraction By Immunoaffinity Column
And The Detection Of Isoforms Using
Capillary Electrophoresis |
title_sort |
recombinant erythropoietin
extraction by immunoaffinity column
and the detection of isoforms using
capillary electrophoresis |
granting_institution |
Universiti Sains Malaysia |
granting_department |
Pusat Penyelidikan Dadah dan Ubat-ubatan |
publishDate |
2013 |
url |
http://eprints.usm.my/43468/1/Hend%20Sultan%20A.S.%20Al-Jaber24.pdf |
_version_ |
1747821221010997248 |