Antihyperglycemic And Toxicological Studies Of Ethanolic–Aqueous Extracts Of Gynura Procumbens

Leaves of Gynura procumbens (sambung nyawa) have been used in Malay traditional medicine among others to treat diabetes. It is understood that the leaves contain many compounds and only one or a few of these compounds possess the antidiabetic activity. Therefore, the aim of this study was to extract...

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Main Author: Algariri, Khaled Saleh
Format: Thesis
Language:English
Published: 2013
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Online Access:http://eprints.usm.my/43526/1/Khaled%20Saleh%20Algariri24.pdf
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spelling my-usm-ep.435262019-04-12T05:26:11Z Antihyperglycemic And Toxicological Studies Of Ethanolic–Aqueous Extracts Of Gynura Procumbens 2013-06 Algariri, Khaled Saleh RS1-441 Pharmacy and materia medica Leaves of Gynura procumbens (sambung nyawa) have been used in Malay traditional medicine among others to treat diabetes. It is understood that the leaves contain many compounds and only one or a few of these compounds possess the antidiabetic activity. Therefore, the aim of this study was to extract the leaves using solvents of different polarity, mixtures of the different ratio of ethanol-water to select the extract that contains compound(s) with the strongest antidiabetic activity and to carry out acute and subchronic toxicity tests of the extract with better activity. The antidiabetic activity of the extracts was compared based on their ability to lower down the blood-glucose level of normal rats and streptozotocin-induced diabetic rats. Dried powdered leaves of the plant were extracted with 0, 25, 50, 75 and 95% ethanol and concentrated using rotary evaporator respectively. The 0, 25, 50, 75 and 95% ethanol extracts respectively significantly reduced (p<0.005) blood-glucose level of streptozotocin induced diabetic rats at 3rd and 5th hours after oral administration of 1000 mg/kg of the extracts. The 25% ethanol extract showed the strongest antidiabetic activity and similar to metformin significantly reduced (p<0.005) blood-glucose level at the 2nd, 3rd, 5th and 7th hours after administration. In the phytochemical analysis the 75% ethanolic extract contained the highest flavonoids content, while the water extract showed the lowest value. In addition, the n-butanol fraction from 25% ethanolic extract represented the highest value of flavonoids and phenolics contents. 2013-06 Thesis http://eprints.usm.my/43526/ http://eprints.usm.my/43526/1/Khaled%20Saleh%20Algariri24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RS1-441 Pharmacy and materia medica
spellingShingle RS1-441 Pharmacy and materia medica
Algariri, Khaled Saleh
Antihyperglycemic And Toxicological Studies Of Ethanolic–Aqueous Extracts Of Gynura Procumbens
description Leaves of Gynura procumbens (sambung nyawa) have been used in Malay traditional medicine among others to treat diabetes. It is understood that the leaves contain many compounds and only one or a few of these compounds possess the antidiabetic activity. Therefore, the aim of this study was to extract the leaves using solvents of different polarity, mixtures of the different ratio of ethanol-water to select the extract that contains compound(s) with the strongest antidiabetic activity and to carry out acute and subchronic toxicity tests of the extract with better activity. The antidiabetic activity of the extracts was compared based on their ability to lower down the blood-glucose level of normal rats and streptozotocin-induced diabetic rats. Dried powdered leaves of the plant were extracted with 0, 25, 50, 75 and 95% ethanol and concentrated using rotary evaporator respectively. The 0, 25, 50, 75 and 95% ethanol extracts respectively significantly reduced (p<0.005) blood-glucose level of streptozotocin induced diabetic rats at 3rd and 5th hours after oral administration of 1000 mg/kg of the extracts. The 25% ethanol extract showed the strongest antidiabetic activity and similar to metformin significantly reduced (p<0.005) blood-glucose level at the 2nd, 3rd, 5th and 7th hours after administration. In the phytochemical analysis the 75% ethanolic extract contained the highest flavonoids content, while the water extract showed the lowest value. In addition, the n-butanol fraction from 25% ethanolic extract represented the highest value of flavonoids and phenolics contents.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Algariri, Khaled Saleh
author_facet Algariri, Khaled Saleh
author_sort Algariri, Khaled Saleh
title Antihyperglycemic And Toxicological Studies Of Ethanolic–Aqueous Extracts Of Gynura Procumbens
title_short Antihyperglycemic And Toxicological Studies Of Ethanolic–Aqueous Extracts Of Gynura Procumbens
title_full Antihyperglycemic And Toxicological Studies Of Ethanolic–Aqueous Extracts Of Gynura Procumbens
title_fullStr Antihyperglycemic And Toxicological Studies Of Ethanolic–Aqueous Extracts Of Gynura Procumbens
title_full_unstemmed Antihyperglycemic And Toxicological Studies Of Ethanolic–Aqueous Extracts Of Gynura Procumbens
title_sort antihyperglycemic and toxicological studies of ethanolic–aqueous extracts of gynura procumbens
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Farmasi
publishDate 2013
url http://eprints.usm.my/43526/1/Khaled%20Saleh%20Algariri24.pdf
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