Morphological Identification, Molecular Characterization And Pathogenicity Of Fusarium Species On Chilli Pepper In Tsunami-Affected Areas, Aceh Province, Indonesia
Chilli pepper (Capsicum annuum) is a high value vegetable crop in Aceh and other parts of Indonesia and Malaysia. The fruits are widely used as vegetables, spices, and medicines worldwide. There are several economically important diseases caused by Fusarium species have been reported on this crop, s...
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| Format: | Thesis |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://eprints.usm.my/43576/1/Bintra%20Mailina24.pdf |
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| Summary: | Chilli pepper (Capsicum annuum) is a high value vegetable crop in Aceh and other parts of Indonesia and Malaysia. The fruits are widely used as vegetables, spices, and medicines worldwide. There are several economically important diseases caused by Fusarium species have been reported on this crop, such as damping-off, wilt, root rot, stem rot, and fruit rot. In the tsunami-affected fields of Aceh Province, two of the disease symptoms were commonly observed, namely wilts and necrotic lesions that were suspected to be caused by Fusarium species. In this study, Fusarium isolates associated with chilli pepper in tsunami-affected areas (Aceh) and non-tsunami affected areas in Indonesia and Peninsular Malaysia (as a comparison) were isolated, identified, characterized and tested for pathogenicity. Identification into species level was conducted based on morphological characters on cultures grown on PDA (Potato Dextrose Agar) and CLA (Carnation Leaf Agar), and further characterized by RAPD analysis. A total of 503 isolates of Fusarium obtained from soil-debris and diseased chilli pepper plants from all sampling areas were identified into eight species, F. semitectum, F. solani, F. proliferatum, F. oxysporum, F. dimerum, F. equiseti, F. subglutinans, and F. sterilihyphosum. The difference frequency of distribution and diversity of Fusarium species were clearly observed between tsunami-affected and non-tsunami affected areas. However, for morphological characters within species, it was shown that the species characters were similar in both tsunami-affected and non-tsunami affected areas. |
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