Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library

Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library

CD36 is a class B scavenger receptor which is involved significantly in the uptake of oxidised low-density lipoprotein (oxLDL). Accumulation of lipid contributes to foam cells formation, which is the precursor to atherosclerosis plaque. The aim of this study was to identify specific peptides which i...

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Main Author: Ayob, Muhammad Salahuddin
Format: Thesis
Language:English
Published: 2018
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/43701/1/MUHAMMAD%20SALAHUDDIN%20%20AYOB.pdf
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spelling my-usm-ep.437012019-04-12T05:24:52Z Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library 2018-01 Ayob, Muhammad Salahuddin R5-920 Medicine (General) CD36 is a class B scavenger receptor which is involved significantly in the uptake of oxidised low-density lipoprotein (oxLDL). Accumulation of lipid contributes to foam cells formation, which is the precursor to atherosclerosis plaque. The aim of this study was to identify specific peptides which interact and bind to CD36 as it has the potential to be developed into a peptide inhibitor and as a molecular intervention in treating athero-inflammatory disorders. In this study, 12-mer Ph.D-12TM Phage Display Peptide Library, which was validated in streptavidin panning, was used in selection against CD36. CD36-binding assay was performed by incubating the selected phages with two CD36-expressing cell lines, CHO-CD36 and U937 and a CD36-non expressing cell line, HeLa, followed by anti-M13 phage-PE and mouse IgG2b-PE isotype antibodies staining. 2018-01 Thesis http://eprints.usm.my/43701/ http://eprints.usm.my/43701/1/MUHAMMAD%20SALAHUDDIN%20%20AYOB.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Ayob, Muhammad Salahuddin
Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library
description CD36 is a class B scavenger receptor which is involved significantly in the uptake of oxidised low-density lipoprotein (oxLDL). Accumulation of lipid contributes to foam cells formation, which is the precursor to atherosclerosis plaque. The aim of this study was to identify specific peptides which interact and bind to CD36 as it has the potential to be developed into a peptide inhibitor and as a molecular intervention in treating athero-inflammatory disorders. In this study, 12-mer Ph.D-12TM Phage Display Peptide Library, which was validated in streptavidin panning, was used in selection against CD36. CD36-binding assay was performed by incubating the selected phages with two CD36-expressing cell lines, CHO-CD36 and U937 and a CD36-non expressing cell line, HeLa, followed by anti-M13 phage-PE and mouse IgG2b-PE isotype antibodies staining.
format Thesis
qualification_level Master's degree
author Ayob, Muhammad Salahuddin
author_facet Ayob, Muhammad Salahuddin
author_sort Ayob, Muhammad Salahuddin
title Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library
title_short Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library
title_full Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library
title_fullStr Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library
title_full_unstemmed Identification Of A Cd36 Scavenger Receptor Binding Peptide Using A 12-Mer Peptide M13 Phage Display Library
title_sort identification of a cd36 scavenger receptor binding peptide using a 12-mer peptide m13 phage display library
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2018
url http://eprints.usm.my/43701/1/MUHAMMAD%20SALAHUDDIN%20%20AYOB.pdf
_version_ 1747821263269658624

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