In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment

Fifteen strains of Lactobacillus and Bifidobacterium were screened based on their adherence property. Lactobacillus acidophilus BT 1088, L. acidophilus FTCC 0291, L. bulgaricus FTCC 0411, L. bulgaricus FTDC 1311, and L. casei BT 1268 showed higher adherence property compared to other strains s...

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Main Author:	Lye, Huey Shi
Format:	Thesis
Language:	English
Published:	2013
Subjects:	T11.95-12.5 Industrial directories
Online Access:	http://eprints.usm.my/43787/1/Lye%20Huey%20Shi24.pdf
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id	my-usm-ep.43787
record_format	uketd_dc
spelling	my-usm-ep.437872019-04-12T05:26:16Z In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment 2013-05 Lye, Huey Shi T11.95-12.5 Industrial directories Fifteen strains of Lactobacillus and Bifidobacterium were screened based on their adherence property. Lactobacillus acidophilus BT 1088, L. acidophilus FTCC 0291, L. bulgaricus FTCC 0411, L. bulgaricus FTDC 1311, and L. casei BT 1268 showed higher adherence property compared to other strains studied and were thus selected for examination on cholesterol removal. Cholesterol removal ability was conducted in vitro, under conditions that mimic the human gastrointestinal tract (pH 8.0). This study provided experimental evidence to strengthen the hypothesis that lactobacilli could remove cholesterol via different mechanisms, namely assimilation of cholesterol during growth, binding of cholesterol to cellular surface, disruption of cholesterol micelle, incorporation of cholesterol into the cellular membrane, deconjugation of bile salt, bile salt hydrolase (BSH) activity, and conversion of cholesterol to coprostanol. Among the mechanisms studied, cholesterol incorporation mechanism was more prominent and the locations of incorporated cholesterol have also been identified. Considering that incorporation of cholesterol into the cellular membrane involves membrane permeability, thus sub-lethal physical treatments such as ultrasound (20-100 W; 1-3 min), electroporation (2.5-7.5 kV cm-1; 3-4 ms), and UV radiation (UVA-UVC, 30-90 J m-2) were applied with the objective to further increase cholesterol removal by Lactobacillus species. 2013-05 Thesis http://eprints.usm.my/43787/ http://eprints.usm.my/43787/1/Lye%20Huey%20Shi24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T11.95-12.5 Industrial directories
spellingShingle	T11.95-12.5 Industrial directories Lye, Huey Shi In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment
description	Fifteen strains of Lactobacillus and Bifidobacterium were screened based on their adherence property. Lactobacillus acidophilus BT 1088, L. acidophilus FTCC 0291, L. bulgaricus FTCC 0411, L. bulgaricus FTDC 1311, and L. casei BT 1268 showed higher adherence property compared to other strains studied and were thus selected for examination on cholesterol removal. Cholesterol removal ability was conducted in vitro, under conditions that mimic the human gastrointestinal tract (pH 8.0). This study provided experimental evidence to strengthen the hypothesis that lactobacilli could remove cholesterol via different mechanisms, namely assimilation of cholesterol during growth, binding of cholesterol to cellular surface, disruption of cholesterol micelle, incorporation of cholesterol into the cellular membrane, deconjugation of bile salt, bile salt hydrolase (BSH) activity, and conversion of cholesterol to coprostanol. Among the mechanisms studied, cholesterol incorporation mechanism was more prominent and the locations of incorporated cholesterol have also been identified. Considering that incorporation of cholesterol into the cellular membrane involves membrane permeability, thus sub-lethal physical treatments such as ultrasound (20-100 W; 1-3 min), electroporation (2.5-7.5 kV cm-1; 3-4 ms), and UV radiation (UVA-UVC, 30-90 J m-2) were applied with the objective to further increase cholesterol removal by Lactobacillus species.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Lye, Huey Shi
author_facet	Lye, Huey Shi
author_sort	Lye, Huey Shi
title	In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment
title_short	In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment
title_full	In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment
title_fullStr	In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment
title_full_unstemmed	In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment
title_sort	in vitro cholesterol-lowering mechanisms of selected lactobacillus and bifidobacterium species and effects of physical treatment
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2013
url	http://eprints.usm.my/43787/1/Lye%20Huey%20Shi24.pdf
_version_	1747821279560335360

In Vitro Cholesterol-Lowering Mechanisms Of Selected Lactobacillus And Bifidobacterium Species And Effects Of Physical Treatment

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