Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection

Everyday inventions of microelectronics and micro-devices are advancing towards converting the Microchip Capillary Electrophoresis (MCE) devices into a true Micro Total Analysis System (μTAS) or Lab-On-a-Chip (LOC) microsystem that can perform the whole analytical procedures in-situ. Electrochemical...

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Main Author:	Ghanim, Motasem H H
Format:	Thesis
Language:	English
Published:	2013
Subjects:	TK1-9971 Electrical engineering Electronics Nuclear engineering
Online Access:	http://eprints.usm.my/43838/1/Motasem%20H%20H%20Ghanim24.pdf
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id	my-usm-ep.43838
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spelling	my-usm-ep.438382019-04-12T05:26:10Z Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection 2013-08 Ghanim, Motasem H H TK1-9971 Electrical engineering. Electronics. Nuclear engineering Everyday inventions of microelectronics and micro-devices are advancing towards converting the Microchip Capillary Electrophoresis (MCE) devices into a true Micro Total Analysis System (μTAS) or Lab-On-a-Chip (LOC) microsystem that can perform the whole analytical procedures in-situ. Electrochemical detection (ECD) is one of the best means for designing a LOC or μTAS systems especially for separation and detection of electroactive analytes, as in biomedical and in vitro diagnostics. The most widely targeted electroactive analytes is nucleic acid. DNA genotyping or fingerprinting has been recognized as one of the most important applications of these micro devices. DNA fingerprinting is normally used in electrophoretic applications of analytical chemistry such as DNA sequencing and detection, identification of pathogenic microorganism like microbe fungus, and disease diagnostics. However, there are some major obstacles in realizing miniaturized and mass-producible MCE systems, such as high manufacturing cost, and the bulky power supply unit which make the whole system none-portable. In this project, a low cost as well as low electric field MCE utilizing an amperometric detection scheme was designed and fabricated for DNA sequencing and detection. The device was fabricated from a glass/polydimethylsiloxane (PDMS) hybrid engraved microchannel with platinum electrodes sputtered onto a glass substrate. It has been established that, the use of Agarose gel as separation material could reduce the electric field to as low as 12 V/cm; this has not been achieved previously. 2013-08 Thesis http://eprints.usm.my/43838/ http://eprints.usm.my/43838/1/Motasem%20H%20H%20Ghanim24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Elektrik & Elektronik
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TK1-9971 Electrical engineering Electronics Nuclear engineering
spellingShingle	TK1-9971 Electrical engineering Electronics Nuclear engineering Ghanim, Motasem H H Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection
description	Everyday inventions of microelectronics and micro-devices are advancing towards converting the Microchip Capillary Electrophoresis (MCE) devices into a true Micro Total Analysis System (μTAS) or Lab-On-a-Chip (LOC) microsystem that can perform the whole analytical procedures in-situ. Electrochemical detection (ECD) is one of the best means for designing a LOC or μTAS systems especially for separation and detection of electroactive analytes, as in biomedical and in vitro diagnostics. The most widely targeted electroactive analytes is nucleic acid. DNA genotyping or fingerprinting has been recognized as one of the most important applications of these micro devices. DNA fingerprinting is normally used in electrophoretic applications of analytical chemistry such as DNA sequencing and detection, identification of pathogenic microorganism like microbe fungus, and disease diagnostics. However, there are some major obstacles in realizing miniaturized and mass-producible MCE systems, such as high manufacturing cost, and the bulky power supply unit which make the whole system none-portable. In this project, a low cost as well as low electric field MCE utilizing an amperometric detection scheme was designed and fabricated for DNA sequencing and detection. The device was fabricated from a glass/polydimethylsiloxane (PDMS) hybrid engraved microchannel with platinum electrodes sputtered onto a glass substrate. It has been established that, the use of Agarose gel as separation material could reduce the electric field to as low as 12 V/cm; this has not been achieved previously.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ghanim, Motasem H H
author_facet	Ghanim, Motasem H H
author_sort	Ghanim, Motasem H H
title	Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection
title_short	Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection
title_full	Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection
title_fullStr	Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection
title_full_unstemmed	Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection
title_sort	development of a low electric field dna electrophoresis microchip featuring amperometric detection
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Elektrik & Elektronik
publishDate	2013
url	http://eprints.usm.my/43838/1/Motasem%20H%20H%20Ghanim24.pdf
_version_	1747821288112521216

Development Of A Low Electric Field Dna Electrophoresis Microchip Featuring Amperometric Detection

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