Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr

The outbreak of influenza A viruses (H1N1 and H5N1) has raised a global concern on the future risk of a pandemic. Oseltamivir, the current neuraminidase inhibitor, could not meet the demand if there is a major outbreak. Thus, there is a need to find alternative treatment for influenza A. Our m...

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Main Author:	Purwitasari, Neny
Format:	Thesis
Language:	English
Published:	2013
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/43863/1/Neny%20Purwitasari24.pdf
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id	my-usm-ep.43863
record_format	uketd_dc
spelling	my-usm-ep.438632019-04-12T05:26:17Z Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr 2013-04 Purwitasari, Neny RS1-441 Pharmacy and materia medica The outbreak of influenza A viruses (H1N1 and H5N1) has raised a global concern on the future risk of a pandemic. Oseltamivir, the current neuraminidase inhibitor, could not meet the demand if there is a major outbreak. Thus, there is a need to find alternative treatment for influenza A. Our molecular docking work on neuraminidase has led to the selection of plant to be studied, Brucea javanica (L.) Merr (Simaroubaceae). The objectives of this study were to determine the neuraminidase inhibition and in vitro influenza A antiviral activities of Brucea javanica (L.) Merr. In order to obtain information about the active compounds from this plant, the dried fruits were extracted using methanol, and fractionated using n-hexane, ethyl acetate and nbutanol. One compound was isolated from ethyl acetate fraction, identified as β- sitosterol 3-O-β-D-glucopyranoside or daucosterol. Crude methanol extract, n-hexane fraction, ethyl acetate fraction, n-butanol fraction as well as daucosterol were subjected to the neuraminidase inhibition assay (MUNANA Assay) against Clostridium perfringens, H5N1 and H1N1 neuraminidase enzymes and in vitro antiviral assay against A/Indonesia/Unair/2011 (H1N1) virus on MDCK cells. The reduction of hemagglutinin titre was performed in hemagglutination assay (HA Assay), while the reduction of virus infectious titre was performed in Half Tissue Culture Infectious Dose (TCID50) assay. 2013-04 Thesis http://eprints.usm.my/43863/ http://eprints.usm.my/43863/1/Neny%20Purwitasari24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Purwitasari, Neny Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr
description	The outbreak of influenza A viruses (H1N1 and H5N1) has raised a global concern on the future risk of a pandemic. Oseltamivir, the current neuraminidase inhibitor, could not meet the demand if there is a major outbreak. Thus, there is a need to find alternative treatment for influenza A. Our molecular docking work on neuraminidase has led to the selection of plant to be studied, Brucea javanica (L.) Merr (Simaroubaceae). The objectives of this study were to determine the neuraminidase inhibition and in vitro influenza A antiviral activities of Brucea javanica (L.) Merr. In order to obtain information about the active compounds from this plant, the dried fruits were extracted using methanol, and fractionated using n-hexane, ethyl acetate and nbutanol. One compound was isolated from ethyl acetate fraction, identified as β- sitosterol 3-O-β-D-glucopyranoside or daucosterol. Crude methanol extract, n-hexane fraction, ethyl acetate fraction, n-butanol fraction as well as daucosterol were subjected to the neuraminidase inhibition assay (MUNANA Assay) against Clostridium perfringens, H5N1 and H1N1 neuraminidase enzymes and in vitro antiviral assay against A/Indonesia/Unair/2011 (H1N1) virus on MDCK cells. The reduction of hemagglutinin titre was performed in hemagglutination assay (HA Assay), while the reduction of virus infectious titre was performed in Half Tissue Culture Infectious Dose (TCID50) assay.
format	Thesis
qualification_level	Master's degree
author	Purwitasari, Neny
author_facet	Purwitasari, Neny
author_sort	Purwitasari, Neny
title	Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr
title_short	Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr
title_full	Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr
title_fullStr	Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr
title_full_unstemmed	Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr
title_sort	neuraminidase inhibition and in vitro anti influenza a (h1n1) activities of brucea javanica (l.) merr
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2013
url	http://eprints.usm.my/43863/1/Neny%20Purwitasari24.pdf
_version_	1747821292024758272

Neuraminidase Inhibition And In Vitro Anti Influenza A (H1n1) Activities Of Brucea Javanica (L.) Merr

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