Diversity Of Fusarium Species In Peat Soils

The occurrence and diversity of Fusarium species were determined from 23 peat soil samples collected from peat swamp forest, water-logged peat and peat soils from oil palm plantations. From soil analysis, the peat soils were mostly sandy and loamy sand, acidic (pH 3-4) with low nitrogen and carbon c...

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Main Author:	Karim, Nurul Farah Abd
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/43906/1/Nurul%20Farah%20Binti%20Abd%20Karim24.pdf
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id	my-usm-ep.43906
record_format	uketd_dc
spelling	my-usm-ep.439062019-04-12T05:26:17Z Diversity Of Fusarium Species In Peat Soils 2013-04 Karim, Nurul Farah Abd QH1 Natural history (General - Including nature conservation, geographical distribution) The occurrence and diversity of Fusarium species were determined from 23 peat soil samples collected from peat swamp forest, water-logged peat and peat soils from oil palm plantations. From soil analysis, the peat soils were mostly sandy and loamy sand, acidic (pH 3-4) with low nitrogen and carbon content and low moisture content. Based on the morphological characteristics of macroconidia, microconidia, conidiogenous cells, colony appearance and pigmentation, five Fusarium species were identified namely, F. oxysporum (60%), F. solani (23%), F. proliferatum (14%), F. semitectum (1%) and F. verticillioides (1%). These species are widely distributed worldwide and are common soil inhabitants which act as saprophyte and decomposer. Species identity was confirmed through DNA sequencing of translation elongation factor (TEF-1α). For species from Gibberella fujikuroi species complex, F. verticillioides and F. proliferatum, mating study was conducted. Mating study results showed that nine isolates of F. proliferatum and two isolates of F. verticillioides carried MAT 2 allele. Cross fertility test indicated that nine morphologically identified F. proliferatum were confirmed as F. proliferatum after cross-fertile with mating population D (Gibberella intermedia) and only one isolate was confirmed as F. verticillioides (Gibberella moniliforme) after cross-fertile with mating population A. From phylogenetic analysis using TEF-1α and β-tubulin genes based on individual dataset and combined dataset using neigbour-joining (NJ) and maximum likelihood (ML) methods, showed that the isolates from the same species were clustered in the same clade. 2013-04 Thesis http://eprints.usm.my/43906/ http://eprints.usm.my/43906/1/Nurul%20Farah%20Binti%20Abd%20Karim24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Karim, Nurul Farah Abd Diversity Of Fusarium Species In Peat Soils
description	The occurrence and diversity of Fusarium species were determined from 23 peat soil samples collected from peat swamp forest, water-logged peat and peat soils from oil palm plantations. From soil analysis, the peat soils were mostly sandy and loamy sand, acidic (pH 3-4) with low nitrogen and carbon content and low moisture content. Based on the morphological characteristics of macroconidia, microconidia, conidiogenous cells, colony appearance and pigmentation, five Fusarium species were identified namely, F. oxysporum (60%), F. solani (23%), F. proliferatum (14%), F. semitectum (1%) and F. verticillioides (1%). These species are widely distributed worldwide and are common soil inhabitants which act as saprophyte and decomposer. Species identity was confirmed through DNA sequencing of translation elongation factor (TEF-1α). For species from Gibberella fujikuroi species complex, F. verticillioides and F. proliferatum, mating study was conducted. Mating study results showed that nine isolates of F. proliferatum and two isolates of F. verticillioides carried MAT 2 allele. Cross fertility test indicated that nine morphologically identified F. proliferatum were confirmed as F. proliferatum after cross-fertile with mating population D (Gibberella intermedia) and only one isolate was confirmed as F. verticillioides (Gibberella moniliforme) after cross-fertile with mating population A. From phylogenetic analysis using TEF-1α and β-tubulin genes based on individual dataset and combined dataset using neigbour-joining (NJ) and maximum likelihood (ML) methods, showed that the isolates from the same species were clustered in the same clade.
format	Thesis
qualification_level	Master's degree
author	Karim, Nurul Farah Abd
author_facet	Karim, Nurul Farah Abd
author_sort	Karim, Nurul Farah Abd
title	Diversity Of Fusarium Species In Peat Soils
title_short	Diversity Of Fusarium Species In Peat Soils
title_full	Diversity Of Fusarium Species In Peat Soils
title_fullStr	Diversity Of Fusarium Species In Peat Soils
title_full_unstemmed	Diversity Of Fusarium Species In Peat Soils
title_sort	diversity of fusarium species in peat soils
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2013
url	http://eprints.usm.my/43906/1/Nurul%20Farah%20Binti%20Abd%20Karim24.pdf
_version_	1747821301573091328

Diversity Of Fusarium Species In Peat Soils

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