Development Of New Analytical Methods For Steroid Profiling For Doping Purposes

Development Of New Analytical Methods For Steroid Profiling For Doping Purposes

The steroid profile compounds namely testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5alpha-androstane-3alpha,17beta-diol (5αAdiol), 5beta-androstane-3alpha,17beta-diol (5βAdiol) and the ratio of T to E (T/E)) has been determined in urine of male volunteers who consu...

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Main Author: Manaf, Normaliza Abdul
Format: Thesis
Language:English
Published: 2018
Subjects:
QD1-999 Chemistry
Online Access:http://eprints.usm.my/43993/1/NORMALIZA%20ABDUL%20MANAF.pdf
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spelling my-usm-ep.439932019-04-12T05:24:50Z Development Of New Analytical Methods For Steroid Profiling For Doping Purposes 2018-07 Manaf, Normaliza Abdul QD1-999 Chemistry The steroid profile compounds namely testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5alpha-androstane-3alpha,17beta-diol (5αAdiol), 5beta-androstane-3alpha,17beta-diol (5βAdiol) and the ratio of T to E (T/E)) has been determined in urine of male volunteers who consumed Tongkat Ali (TA) supplement using the solid phase extraction (SPE) and liquid-liquid extraction (LLE) method. From 47 samples, 11 samples violated the World Anti-Doping Agency (WADA) guidelines and confirmation by gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) showed 5 samples were consistent with the exogenous origin (Δδ13C > 3‰). Two microextraction methods, i.e., vortexassisted liquid-liquid microextraction (VALLME) and micro-solid phase extraction (μ-SPE) followed by LC-MS/MS were developed. Hypersil Gold C18 column (50 mm × 2.1 mm, 1.9 μm) with gradient elution resulted in baseline separation for compounds in about 8 min. 2018-07 Thesis http://eprints.usm.my/43993/ http://eprints.usm.my/43993/1/NORMALIZA%20ABDUL%20MANAF.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kimia
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QD1-999 Chemistry
spellingShingle QD1-999 Chemistry
Manaf, Normaliza Abdul
Development Of New Analytical Methods For Steroid Profiling For Doping Purposes
description The steroid profile compounds namely testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5alpha-androstane-3alpha,17beta-diol (5αAdiol), 5beta-androstane-3alpha,17beta-diol (5βAdiol) and the ratio of T to E (T/E)) has been determined in urine of male volunteers who consumed Tongkat Ali (TA) supplement using the solid phase extraction (SPE) and liquid-liquid extraction (LLE) method. From 47 samples, 11 samples violated the World Anti-Doping Agency (WADA) guidelines and confirmation by gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) showed 5 samples were consistent with the exogenous origin (Δδ13C > 3‰). Two microextraction methods, i.e., vortexassisted liquid-liquid microextraction (VALLME) and micro-solid phase extraction (μ-SPE) followed by LC-MS/MS were developed. Hypersil Gold C18 column (50 mm × 2.1 mm, 1.9 μm) with gradient elution resulted in baseline separation for compounds in about 8 min.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Manaf, Normaliza Abdul
author_facet Manaf, Normaliza Abdul
author_sort Manaf, Normaliza Abdul
title Development Of New Analytical Methods For Steroid Profiling For Doping Purposes
title_short Development Of New Analytical Methods For Steroid Profiling For Doping Purposes
title_full Development Of New Analytical Methods For Steroid Profiling For Doping Purposes
title_fullStr Development Of New Analytical Methods For Steroid Profiling For Doping Purposes
title_full_unstemmed Development Of New Analytical Methods For Steroid Profiling For Doping Purposes
title_sort development of new analytical methods for steroid profiling for doping purposes
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kimia
publishDate 2018
url http://eprints.usm.my/43993/1/NORMALIZA%20ABDUL%20MANAF.pdf
_version_ 1747821315015835648

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