Leptospiral Proteins Induced In-Vivo And Its Application In The Development Of Antibody And Antigen Detection Tests For Acute Leptospirosis
Leptospirosis, caused by pathogenic Leptospira spp., is a re-emerging global health threat. Therefore, it is pertinent to identify novel diagnostic marker and investigate its potential use in antibody and antigen detection assays for detection of acute leptospirosis. Using panels of serum samples fr...
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2018
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my-usm-ep.440152019-04-12T05:24:51Z Leptospiral Proteins Induced In-Vivo And Its Application In The Development Of Antibody And Antigen Detection Tests For Acute Leptospirosis 2018-05 Chang, Chiat Han R735-854 Medical education. Medical schools. Research Leptospirosis, caused by pathogenic Leptospira spp., is a re-emerging global health threat. Therefore, it is pertinent to identify novel diagnostic marker and investigate its potential use in antibody and antigen detection assays for detection of acute leptospirosis. Using panels of serum samples from acute (Group I) and mixed (Group II) phase leptospirosis, the present study was initiated by evaluating performance of two leptospirosis rapid diagnostic kits, namely Leptorapide and VISITECT-LEPTO, that were commonly used in Malaysia. Both test kits showed low diagnostic sensitivity (≤34%) with the acute phase serum samples, but better sensitivity with the mixed phase samples. Selected serum samples from Group I were used to identify novel diagnostic marker(s) from Leptospira genomic DNA expression library using In-vivo Induced Antigen Technology. 2018-05 Thesis http://eprints.usm.my/44015/ http://eprints.usm.my/44015/1/CHANG%20CHIAT%20HAN.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul |
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Universiti Sains Malaysia |
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USM Institutional Repository |
| language |
English |
| topic |
R735-854 Medical education Medical schools Research |
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R735-854 Medical education Medical schools Research Chang, Chiat Han Leptospiral Proteins Induced In-Vivo And Its Application In The Development Of Antibody And Antigen Detection Tests For Acute Leptospirosis |
| description |
Leptospirosis, caused by pathogenic Leptospira spp., is a re-emerging global health threat. Therefore, it is pertinent to identify novel diagnostic marker and investigate its potential use in antibody and antigen detection assays for detection of acute leptospirosis. Using panels of serum samples from acute (Group I) and mixed (Group II) phase leptospirosis, the present study was initiated by evaluating performance of two leptospirosis rapid diagnostic kits, namely Leptorapide and VISITECT-LEPTO, that were commonly used in Malaysia. Both test kits showed low diagnostic sensitivity (≤34%) with the acute phase serum samples, but better sensitivity with the mixed phase samples. Selected serum samples from Group I were used to identify novel diagnostic marker(s) from Leptospira genomic DNA expression library using In-vivo Induced Antigen Technology. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Chang, Chiat Han |
| author_facet |
Chang, Chiat Han |
| author_sort |
Chang, Chiat Han |
| title |
Leptospiral Proteins Induced In-Vivo And Its Application In The Development Of Antibody And Antigen Detection Tests For Acute Leptospirosis |
| title_short |
Leptospiral Proteins Induced In-Vivo And Its Application In The Development Of Antibody And Antigen Detection Tests For Acute Leptospirosis |
| title_full |
Leptospiral Proteins Induced In-Vivo And Its Application In The Development Of Antibody And Antigen Detection Tests For Acute Leptospirosis |
| title_fullStr |
Leptospiral Proteins Induced In-Vivo And Its Application In The Development Of Antibody And Antigen Detection Tests For Acute Leptospirosis |
| title_full_unstemmed |
Leptospiral Proteins Induced In-Vivo And Its Application In The Development Of Antibody And Antigen Detection Tests For Acute Leptospirosis |
| title_sort |
leptospiral proteins induced in-vivo and its application in the development of antibody and antigen detection tests for acute leptospirosis |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Institut Penyelidikan Perubatan Molekul |
| publishDate |
2018 |
| url |
http://eprints.usm.my/44015/1/CHANG%20CHIAT%20HAN.pdf |
| _version_ |
1747821319663124480 |