The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells

D6 and DARC had been reported as a decoy chemokine receptor in cancer study. The involvement of D6 and DARC in breast cancer had been investigated and it was reported to negatively correlate with the progression and metastasis of breast cancer cells. This study aimed to construct recombinant clones...

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Main Author:	Tan, Wee Yee
Format:	Thesis
Language:	English
Published:	2018
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/44025/1/TAN%20WEE%20YEE.pdf
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id	my-usm-ep.44025
record_format	uketd_dc
spelling	my-usm-ep.440252019-04-12T05:24:50Z The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells 2018-07 Tan, Wee Yee R735-854 Medical education. Medical schools. Research D6 and DARC had been reported as a decoy chemokine receptor in cancer study. The involvement of D6 and DARC in breast cancer had been investigated and it was reported to negatively correlate with the progression and metastasis of breast cancer cells. This study aimed to construct recombinant clones of D6 and DARC, express, analyze and purify the proteins and then determine the effects of the recombinant proteins on breast cancer cell migration and invasion. D6 and DARC genes in MDA-MB-231 cell line were first amplified by one-step RT-PCR with gene specific primers and Pfu DNA polymerase. Besides, DARC was also amplified by PCR using Phusion DNA polymerase. For each of the clones, two pairs of specific primers were used to generate full length nucleotide sequences which were cloned into pPICZ and pPICZα expression vectors. 2018-07 Thesis http://eprints.usm.my/44025/ http://eprints.usm.my/44025/1/TAN%20WEE%20YEE.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Tan, Wee Yee The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
description	D6 and DARC had been reported as a decoy chemokine receptor in cancer study. The involvement of D6 and DARC in breast cancer had been investigated and it was reported to negatively correlate with the progression and metastasis of breast cancer cells. This study aimed to construct recombinant clones of D6 and DARC, express, analyze and purify the proteins and then determine the effects of the recombinant proteins on breast cancer cell migration and invasion. D6 and DARC genes in MDA-MB-231 cell line were first amplified by one-step RT-PCR with gene specific primers and Pfu DNA polymerase. Besides, DARC was also amplified by PCR using Phusion DNA polymerase. For each of the clones, two pairs of specific primers were used to generate full length nucleotide sequences which were cloned into pPICZ and pPICZα expression vectors.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Tan, Wee Yee
author_facet	Tan, Wee Yee
author_sort	Tan, Wee Yee
title	The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_short	The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_full	The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_fullStr	The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_full_unstemmed	The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells
title_sort	production and purification of d6 and darc chemokine decoy receptor recombinant proteins and their effects on migration and invasion in mda-mb-231 and mcf-7 cells
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2018
url	http://eprints.usm.my/44025/1/TAN%20WEE%20YEE.pdf
_version_	1747821321865134080

The Production And Purification Of D6 And Darc Chemokine Decoy Receptor Recombinant Proteins And Their Effects On Migration And Invasion In Mda-Mb-231 And Mcf-7 Cells

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