Optimization of sugar assimilation test for identification of clinically isolated yeast

The study to developed the sugar assimilation test for identification of clinically isolated yeast was done using the swab lawning method and the method of incorporation of suspension on to medium. The result obtained, showed that the swab lawning method was not feasible to be used for identifyin...

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Main Author: Othman, Afaf Alani Che
Format: Thesis
Language:English
Published: 2004
Subjects:
Online Access:http://eprints.usm.my/44144/1/LI...Afaf%20Alani%20Binti%20Che%20Othman...2004...-24%20pages.pdf
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spelling my-usm-ep.441442020-10-22T03:03:26Z Optimization of sugar assimilation test for identification of clinically isolated yeast 2004 Othman, Afaf Alani Che R Medicine The study to developed the sugar assimilation test for identification of clinically isolated yeast was done using the swab lawning method and the method of incorporation of suspension on to medium. The result obtained, showed that the swab lawning method was not feasible to be used for identifying clinically isolated yeast based on the sugar assimilation test However, the method of incorporation of suspension on medium was comparatively better and was carried out by optimizing the test by varying the sugar concentration, medium pH and incubation temperature. Candida spp. were screened using 14 types of sugars. The optimized conditions for identification purpose was 20% ( w/v) of sugar concentration, medium pH of 5.6 and the incubation temperature of 30°C for both Candida albican and Candida tropicalis. Under these optimized conditions for identification, Candida a/bican did not utilized cellobiose, whereas Candida tropica/is utilized cellobiose. But the optimum growth conditions for both species were same. Therefore the method of incorporation of suspension under the optimized conditions may be used in the identification of clinically isolated yeast especially in differentiating Candida a/bican and Candida tropical is. 2004 Thesis http://eprints.usm.my/44144/ http://eprints.usm.my/44144/1/LI...Afaf%20Alani%20Binti%20Che%20Othman...2004...-24%20pages.pdf application/pdf en public other Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R Medicine
spellingShingle R Medicine
Othman, Afaf Alani Che
Optimization of sugar assimilation test for identification of clinically isolated yeast
description The study to developed the sugar assimilation test for identification of clinically isolated yeast was done using the swab lawning method and the method of incorporation of suspension on to medium. The result obtained, showed that the swab lawning method was not feasible to be used for identifying clinically isolated yeast based on the sugar assimilation test However, the method of incorporation of suspension on medium was comparatively better and was carried out by optimizing the test by varying the sugar concentration, medium pH and incubation temperature. Candida spp. were screened using 14 types of sugars. The optimized conditions for identification purpose was 20% ( w/v) of sugar concentration, medium pH of 5.6 and the incubation temperature of 30°C for both Candida albican and Candida tropicalis. Under these optimized conditions for identification, Candida a/bican did not utilized cellobiose, whereas Candida tropica/is utilized cellobiose. But the optimum growth conditions for both species were same. Therefore the method of incorporation of suspension under the optimized conditions may be used in the identification of clinically isolated yeast especially in differentiating Candida a/bican and Candida tropical is.
format Thesis
qualification_level other
author Othman, Afaf Alani Che
author_facet Othman, Afaf Alani Che
author_sort Othman, Afaf Alani Che
title Optimization of sugar assimilation test for identification of clinically isolated yeast
title_short Optimization of sugar assimilation test for identification of clinically isolated yeast
title_full Optimization of sugar assimilation test for identification of clinically isolated yeast
title_fullStr Optimization of sugar assimilation test for identification of clinically isolated yeast
title_full_unstemmed Optimization of sugar assimilation test for identification of clinically isolated yeast
title_sort optimization of sugar assimilation test for identification of clinically isolated yeast
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kesihatan
publishDate 2004
url http://eprints.usm.my/44144/1/LI...Afaf%20Alani%20Binti%20Che%20Othman...2004...-24%20pages.pdf
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