Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications

This work describes the synthesis of gold nanorods (AuNRs) produced using the citrate seed mediated method and cetyltrimethylammonium bromide (CTAB) seed mediated method. The AuNRs are conjugated with antibody for diagnostic applications. The synthesis of AuNRs via the seed mediated method involves...

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Main Author:	Teoh, Poay Ling
Format:	Thesis
Language:	English
Published:	2012
Subjects:	TN1-997 Mining engineering Metallurgy
Online Access:	http://eprints.usm.my/44184/1/Teoh%20Poay%20Ling_HJ.pdf
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id	my-usm-ep.44184
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spelling	my-usm-ep.441842019-04-23T06:35:30Z Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications 2012-02 Teoh, Poay Ling TN1-997 Mining engineering. Metallurgy This work describes the synthesis of gold nanorods (AuNRs) produced using the citrate seed mediated method and cetyltrimethylammonium bromide (CTAB) seed mediated method. The AuNRs are conjugated with antibody for diagnostic applications. The synthesis of AuNRs via the seed mediated method involves two consecutive stages: preparation of seed solution and growth stage. The AuNRs were formed upon the addition of seed solution into the growth solution. By comparing the yield of AuNRs synthesized from both type of seed solutions, it was found that higher yield and longer AuNRs was produced by using CTAB seed solution. Meanwhile, the properties of AuNRs were also influenced by the synthesis parameters of the growth solution such as concentration of silver nitrate (AgNO3), concentration of CTAB, concentration of ascorbic acid, volume of seed solution and volume of nitric acid (HNO3). Among these samples, the optimum synthesis parameters to produce AuNRs were 0.2 M CTAB, 0.08 M ascorbic acid, 50 l seed solution and 300 μl HNO3 via CTAB seed mediated growth method. The optimum synthesis parameters produced 4.9 aspect ratio of AuNRs. The performance of AuNRs conjugated mouse anti-human IgG4 was successfully checked using an immunochromatographic (ICG) strip assay. From the test strip result, the intensity of the control line increased with increasing the amount of mouse anti-human IgG4. The result showed that the covalent attachment between AuNRs and mouse anti-human IgG4 exhibited the best conjugation. 2012-02 Thesis http://eprints.usm.my/44184/ http://eprints.usm.my/44184/1/Teoh%20Poay%20Ling_HJ.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Bahan & Sumber Mineral
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TN1-997 Mining engineering Metallurgy
spellingShingle	TN1-997 Mining engineering Metallurgy Teoh, Poay Ling Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications
description	This work describes the synthesis of gold nanorods (AuNRs) produced using the citrate seed mediated method and cetyltrimethylammonium bromide (CTAB) seed mediated method. The AuNRs are conjugated with antibody for diagnostic applications. The synthesis of AuNRs via the seed mediated method involves two consecutive stages: preparation of seed solution and growth stage. The AuNRs were formed upon the addition of seed solution into the growth solution. By comparing the yield of AuNRs synthesized from both type of seed solutions, it was found that higher yield and longer AuNRs was produced by using CTAB seed solution. Meanwhile, the properties of AuNRs were also influenced by the synthesis parameters of the growth solution such as concentration of silver nitrate (AgNO3), concentration of CTAB, concentration of ascorbic acid, volume of seed solution and volume of nitric acid (HNO3). Among these samples, the optimum synthesis parameters to produce AuNRs were 0.2 M CTAB, 0.08 M ascorbic acid, 50 l seed solution and 300 μl HNO3 via CTAB seed mediated growth method. The optimum synthesis parameters produced 4.9 aspect ratio of AuNRs. The performance of AuNRs conjugated mouse anti-human IgG4 was successfully checked using an immunochromatographic (ICG) strip assay. From the test strip result, the intensity of the control line increased with increasing the amount of mouse anti-human IgG4. The result showed that the covalent attachment between AuNRs and mouse anti-human IgG4 exhibited the best conjugation.
format	Thesis
qualification_level	Master's degree
author	Teoh, Poay Ling
author_facet	Teoh, Poay Ling
author_sort	Teoh, Poay Ling
title	Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications
title_short	Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications
title_full	Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications
title_fullStr	Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications
title_full_unstemmed	Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications
title_sort	synthesis of gold nanorods using the seed mediated method and its conjugation with antibody for diagnostics applications
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Bahan & Sumber Mineral
publishDate	2012
url	http://eprints.usm.my/44184/1/Teoh%20Poay%20Ling_HJ.pdf
_version_	1747821338971602944

Synthesis Of Gold Nanorods Using The Seed Mediated Method And Its Conjugation With Antibody For Diagnostics Applications

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