Expression of the transcription factor, PPAR in human monocytes

The peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of transcription factor that mediate liganddependent transcriptional activation and repression. They regulate genes associated with lipid and glucose metabolism. Recent evidence suggest...

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Main Author:	Jen, Ku Sheau
Format:	Thesis
Language:	English
Published:	2004
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/44402/1/LI...Ku%20Sheau%20Jen...2004...-24%20pages.pdf
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id	my-usm-ep.44402
record_format	uketd_dc
spelling	my-usm-ep.444022020-10-22T03:03:26Z Expression of the transcription factor, PPAR in human monocytes 2004 Jen, Ku Sheau R Medicine The peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of transcription factor that mediate liganddependent transcriptional activation and repression. They regulate genes associated with lipid and glucose metabolism. Recent evidence suggests that PPARs may also act as a negative immunomodulator. To investigate the potential role of PPARa, y1 and y2 in regulating inflammation mediated by monocyte, the expression of PPARa, y1 and y2 in lipopolysaccharide (LPS)-activated and non-activated human monocytes was quantified. Monocytes secrete inflammatory cytokines such as interleukin (IL)-1 ~' IL-6 and tumor necrosis factor (TNF)-a in response to LPS. To verify stimulation of monocytes by LPS, various cytokines including granulocyte/macrophage colony-stimulating factor (GM-CSF), TN F-a, IL-1 ~' IL-6, IL-8 and transforming growth factor (TGF)J3 expression of LPS-activated and non-activated human monocytes was analyzed by using multiplex PCR and their expression is normalized against glyceraldehyde-3- phosphate dehydrogenase (GAPDH) expression. All these inflammatory cytokine expressions were increased in LPS-activated monocytes compared to non-activated monocytes. Measurement of the gene expression levels of PPARa, PPARy1 and PPARy2 in both LPS-activated and non-activated monocytes was carried out using Real-Time PCR analysis. The study showed that LPS induced expression of both PPARa and PPARy2 in isolated human monocytes with a preferential upregulation of PPARy2. The PPARy1 however was not expressed in both LPS-activated and non-activated 2004 Thesis http://eprints.usm.my/44402/ http://eprints.usm.my/44402/1/LI...Ku%20Sheau%20Jen...2004...-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Jen, Ku Sheau Expression of the transcription factor, PPAR in human monocytes
description	The peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of transcription factor that mediate liganddependent transcriptional activation and repression. They regulate genes associated with lipid and glucose metabolism. Recent evidence suggests that PPARs may also act as a negative immunomodulator. To investigate the potential role of PPARa, y1 and y2 in regulating inflammation mediated by monocyte, the expression of PPARa, y1 and y2 in lipopolysaccharide (LPS)-activated and non-activated human monocytes was quantified. Monocytes secrete inflammatory cytokines such as interleukin (IL)-1 ~' IL-6 and tumor necrosis factor (TNF)-a in response to LPS. To verify stimulation of monocytes by LPS, various cytokines including granulocyte/macrophage colony-stimulating factor (GM-CSF), TN F-a, IL-1 ~' IL-6, IL-8 and transforming growth factor (TGF)J3 expression of LPS-activated and non-activated human monocytes was analyzed by using multiplex PCR and their expression is normalized against glyceraldehyde-3- phosphate dehydrogenase (GAPDH) expression. All these inflammatory cytokine expressions were increased in LPS-activated monocytes compared to non-activated monocytes. Measurement of the gene expression levels of PPARa, PPARy1 and PPARy2 in both LPS-activated and non-activated monocytes was carried out using Real-Time PCR analysis. The study showed that LPS induced expression of both PPARa and PPARy2 in isolated human monocytes with a preferential upregulation of PPARy2. The PPARy1 however was not expressed in both LPS-activated and non-activated
format	Thesis
qualification_level	Master's degree
author	Jen, Ku Sheau
author_facet	Jen, Ku Sheau
author_sort	Jen, Ku Sheau
title	Expression of the transcription factor, PPAR in human monocytes
title_short	Expression of the transcription factor, PPAR in human monocytes
title_full	Expression of the transcription factor, PPAR in human monocytes
title_fullStr	Expression of the transcription factor, PPAR in human monocytes
title_full_unstemmed	Expression of the transcription factor, PPAR in human monocytes
title_sort	expression of the transcription factor, ppar in human monocytes
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2004
url	http://eprints.usm.my/44402/1/LI...Ku%20Sheau%20Jen...2004...-24%20pages.pdf
_version_	1747821370402668544

Expression of the transcription factor, PPAR in human monocytes

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