Evaluation of immune status among regular Malay male whole blood donors

Blood donations are an essential part of healthcare system. It is crucial to ensure that sufficient and safe supplies of blood from blood donation. However, it is important to ensure that the donor‟s health is not compromised by the donation of blood or blood component. Whole blood donors may los...

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Main Author:	Fauzi, Norhayati
Format:	Thesis
Language:	English
Published:	2017
Subjects:	RC554-569.5 Personality disorders Behavior problems Including sexual problems, drug abuse,suicide, child abuse
Online Access:	http://eprints.usm.my/44519/1/Dr%20Norhayati%20Fauzi-24%20pages.pdf
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spelling	my-usm-ep.445192020-10-22T03:03:13Z Evaluation of immune status among regular Malay male whole blood donors 2017 Fauzi, Norhayati RC554-569.5 Personality disorders. Behavior problems Including sexual problems, drug abuse,suicide, child abuse Blood donations are an essential part of healthcare system. It is crucial to ensure that sufficient and safe supplies of blood from blood donation. However, it is important to ensure that the donor‟s health is not compromised by the donation of blood or blood component. Whole blood donors may lose approximately 2x109 leukocytes in a single whole blood donation, with no significant drop in peripheral blood lymphocyte count. The possibility of blood donation may change the immune status should be assessed. This study aimed to evaluate and compare the levels of immune markers among first time and regular whole blood donors, as well as, to determine association between the levels of immune markers with the frequency of whole blood donation. A comparative cross sectional study was done at Hospital USM from May 2015 to April 2016. Peripheral blood was taken from 40 regular and 40 first time; Malay male whole blood donors. The immune marker parameters that were studied included TWBCs with neutrophils, lymphocytes, monocytes, eosinophils and basophils count by using XE 5000 Sysmex haematology analyzer (Koba, Japan). Flow cytometry for percentage and absolute count of CD3, CD4, CD8, CD16+56+, CD19 and CD4:CD8 ratio using BD FACSCANTO II. IL-2, IL-10 and IFNγ level quantification by using ELISA immunoassay. IgG, IgA and IgM level quantification by using nephlometry. The mean (SD) of monocytes absolute count in first time and regular whole blood donors were 0.51 (0.14) and 0.58 (0.16), respectively with p value significantly higher in regular whole blood donors. The mean (SD) of interleukin 10 in first time and regular whole blood donors were 6.60 (3.05) and 5.04 (2.28), respectively with p value significantly lower in regular whole blood donors. The mean (SD) of Ig A in first time and regular whole blood donors were 2.24 (0.89) and 2.71(1.02), respectively with mean value significantly higher in regular whole blood donors. Other parameters showed no significant mean different. Analysis for determination of association between the levels of immune markers with frequency of whole blood donation showed no significant association. This present study demonstrates regular blood donors showed no serious significant effects both for cellular and humoral immune markers when compared with the first time blood donors. The differences in monocytes absolute count, IL-10 and IgA between the two groups are probably related to variation of immune activity with no clinical significance. Furthermore, there was no significant association between the levels of immune markers with frequency of whole blood donation. Within limitations of this present study, there are no added risks to regular blood donors as a result of immune changes. 2017 Thesis http://eprints.usm.my/44519/ http://eprints.usm.my/44519/1/Dr%20Norhayati%20Fauzi-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC554-569.5 Personality disorders Behavior problems Including sexual problems, drug abuse,suicide, child abuse
spellingShingle	RC554-569.5 Personality disorders Behavior problems Including sexual problems, drug abuse,suicide, child abuse Fauzi, Norhayati Evaluation of immune status among regular Malay male whole blood donors
description	Blood donations are an essential part of healthcare system. It is crucial to ensure that sufficient and safe supplies of blood from blood donation. However, it is important to ensure that the donor‟s health is not compromised by the donation of blood or blood component. Whole blood donors may lose approximately 2x109 leukocytes in a single whole blood donation, with no significant drop in peripheral blood lymphocyte count. The possibility of blood donation may change the immune status should be assessed. This study aimed to evaluate and compare the levels of immune markers among first time and regular whole blood donors, as well as, to determine association between the levels of immune markers with the frequency of whole blood donation. A comparative cross sectional study was done at Hospital USM from May 2015 to April 2016. Peripheral blood was taken from 40 regular and 40 first time; Malay male whole blood donors. The immune marker parameters that were studied included TWBCs with neutrophils, lymphocytes, monocytes, eosinophils and basophils count by using XE 5000 Sysmex haematology analyzer (Koba, Japan). Flow cytometry for percentage and absolute count of CD3, CD4, CD8, CD16+56+, CD19 and CD4:CD8 ratio using BD FACSCANTO II. IL-2, IL-10 and IFNγ level quantification by using ELISA immunoassay. IgG, IgA and IgM level quantification by using nephlometry. The mean (SD) of monocytes absolute count in first time and regular whole blood donors were 0.51 (0.14) and 0.58 (0.16), respectively with p value significantly higher in regular whole blood donors. The mean (SD) of interleukin 10 in first time and regular whole blood donors were 6.60 (3.05) and 5.04 (2.28), respectively with p value significantly lower in regular whole blood donors. The mean (SD) of Ig A in first time and regular whole blood donors were 2.24 (0.89) and 2.71(1.02), respectively with mean value significantly higher in regular whole blood donors. Other parameters showed no significant mean different. Analysis for determination of association between the levels of immune markers with frequency of whole blood donation showed no significant association. This present study demonstrates regular blood donors showed no serious significant effects both for cellular and humoral immune markers when compared with the first time blood donors. The differences in monocytes absolute count, IL-10 and IgA between the two groups are probably related to variation of immune activity with no clinical significance. Furthermore, there was no significant association between the levels of immune markers with frequency of whole blood donation. Within limitations of this present study, there are no added risks to regular blood donors as a result of immune changes.
format	Thesis
qualification_level	Master's degree
author	Fauzi, Norhayati
author_facet	Fauzi, Norhayati
author_sort	Fauzi, Norhayati
title	Evaluation of immune status among regular Malay male whole blood donors
title_short	Evaluation of immune status among regular Malay male whole blood donors
title_full	Evaluation of immune status among regular Malay male whole blood donors
title_fullStr	Evaluation of immune status among regular Malay male whole blood donors
title_full_unstemmed	Evaluation of immune status among regular Malay male whole blood donors
title_sort	evaluation of immune status among regular malay male whole blood donors
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2017
url	http://eprints.usm.my/44519/1/Dr%20Norhayati%20Fauzi-24%20pages.pdf
_version_	1747821380666130432

Evaluation of immune status among regular Malay male whole blood donors

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