Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28

Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs...

Full description

Saved in:
Bibliographic Details
Main Author: Julkifle, Advina Lizah
Format: Thesis
Language:English
Published: 2012
Subjects:
Online Access:http://eprints.usm.my/44776/1/ADVINA%20LIZAH%20BINTI%20JULKIFLE.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-usm-ep.44776
record_format uketd_dc
spelling my-usm-ep.447762019-07-01T02:11:17Z Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28 2012-06 Julkifle, Advina Lizah QH1 Natural history (General - Including nature conservation, geographical distribution) Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid and liquid cultures. In sucrose treatment, the highest PLBs growth rate percentage was in 10 g.L-1 sucrose. In the conditions of absence of sucrose (0 g.L-1) or excessive of sucrose (30 g.L-1) had proven detrimental for the PLBs growth and development. 2012-06 Thesis http://eprints.usm.my/44776/ http://eprints.usm.my/44776/1/ADVINA%20LIZAH%20BINTI%20JULKIFLE.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1 Natural history (General - Including nature conservation
geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation
geographical distribution)
Julkifle, Advina Lizah
Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
description Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved by using micropropagation technique. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid and liquid cultures. In sucrose treatment, the highest PLBs growth rate percentage was in 10 g.L-1 sucrose. In the conditions of absence of sucrose (0 g.L-1) or excessive of sucrose (30 g.L-1) had proven detrimental for the PLBs growth and development.
format Thesis
qualification_level Master's degree
author Julkifle, Advina Lizah
author_facet Julkifle, Advina Lizah
author_sort Julkifle, Advina Lizah
title Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
title_short Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
title_full Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
title_fullStr Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
title_full_unstemmed Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
title_sort agrobacterium-mediated transformation of dendrobium sonia-28
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2012
url http://eprints.usm.my/44776/1/ADVINA%20LIZAH%20BINTI%20JULKIFLE.pdf
_version_ 1747821396109557760