Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System

Curcuma aeruginosa and C. heyneana aseptic rhizomatous bud explants were established with immersion in 70% (v/v) ethanol for 10 minutes followed by surface-sterilization with 20% (v/v) Clorox® for 20 minutes. This surface-sterilization technique enabled 73.7% and 83.3% of C. aeruginosa and C. heynea...

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Main Author:	., Novianti Suwitosari
Format:	Thesis
Language:	English
Published:	2012
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/44871/1/NOVIANTI%20SUWITOSARI.pdf
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id	my-usm-ep.44871
record_format	uketd_dc
spelling	my-usm-ep.448712019-07-04T08:11:18Z Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System 2012-06 ., Novianti Suwitosari QH1 Natural history (General - Including nature conservation, geographical distribution) Curcuma aeruginosa and C. heyneana aseptic rhizomatous bud explants were established with immersion in 70% (v/v) ethanol for 10 minutes followed by surface-sterilization with 20% (v/v) Clorox® for 20 minutes. This surface-sterilization technique enabled 73.7% and 83.3% of C. aeruginosa and C. heyneana bud explants became aseptic with 66.7% and 87.5% survived explants, respectively. All the aseptic explants when cultured on MS medium supplemented with 2.0 mg/l BA and 0.5 mg/l NAA produced 1.9 shoots per explant for C. aeruginosa and C. heyneana produced 2.1 shoots per explant after four weeks of culture. The shoot explants of C. aeruginosa subsequently produced more multiple shoots when cultured in liquid shoot proliferation medium, while the number of shoots produced from C. heyneana was found to be no different in the gelled or liquid medium. 2012-06 Thesis http://eprints.usm.my/44871/ http://eprints.usm.my/44871/1/NOVIANTI%20SUWITOSARI.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) ., Novianti Suwitosari Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System
description	Curcuma aeruginosa and C. heyneana aseptic rhizomatous bud explants were established with immersion in 70% (v/v) ethanol for 10 minutes followed by surface-sterilization with 20% (v/v) Clorox® for 20 minutes. This surface-sterilization technique enabled 73.7% and 83.3% of C. aeruginosa and C. heyneana bud explants became aseptic with 66.7% and 87.5% survived explants, respectively. All the aseptic explants when cultured on MS medium supplemented with 2.0 mg/l BA and 0.5 mg/l NAA produced 1.9 shoots per explant for C. aeruginosa and C. heyneana produced 2.1 shoots per explant after four weeks of culture. The shoot explants of C. aeruginosa subsequently produced more multiple shoots when cultured in liquid shoot proliferation medium, while the number of shoots produced from C. heyneana was found to be no different in the gelled or liquid medium.
format	Thesis
qualification_level	Master's degree
author	., Novianti Suwitosari
author_facet	., Novianti Suwitosari
author_sort	., Novianti Suwitosari
title	Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System
title_short	Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System
title_full	Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System
title_fullStr	Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System
title_full_unstemmed	Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System
title_sort	micropropagation of curcuma aeruginosa and curcuma heyneana using aerated culture system
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2012
url	http://eprints.usm.my/44871/1/NOVIANTI%20SUWITOSARI.pdf
_version_	1747821416327151616

Micropropagation Of Curcuma Aeruginosa And Curcuma Heyneana Using Aerated Culture System

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