Screening For Small Molecule Inhibitors Of p27Kip1 Degradation

Screening For Small Molecule Inhibitors Of p27Kip1 Degradation

In a majority of human cancers, the cyclin-dependent kinase (CDK) inhibitor p27Kip1 is commonly found to be deregulated due to proteolysis by the ubiquitin-proteasome pathway involving the SCFSkp2 E3 ligase. Although proteasome inhibitors act to stabilize p27Kip1, small molecules agents inhibiting t...

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Main Author: Ooi, Li Ching
Format: Thesis
Language:English
Published: 2012
Subjects:
QH1 Natural history (General - Including nature conservation
geographical distribution)
Online Access:http://eprints.usm.my/44880/1/OOI%20LI%20CHING.pdf
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spelling my-usm-ep.448802019-07-05T01:56:11Z Screening For Small Molecule Inhibitors Of p27Kip1 Degradation 2012-07 Ooi, Li Ching QH1 Natural history (General - Including nature conservation, geographical distribution) In a majority of human cancers, the cyclin-dependent kinase (CDK) inhibitor p27Kip1 is commonly found to be deregulated due to proteolysis by the ubiquitin-proteasome pathway involving the SCFSkp2 E3 ligase. Although proteasome inhibitors act to stabilize p27Kip1, small molecules agents inhibiting the degradation of p27Kip1 by the function of SCFSkp2 E3 ligase may contribute to the development of a specific targeted drug that could prove to be more efficacious with less side effects. In this work, we have developed a high-throughput screening system based on phosphorylation dependent protein-protein interaction using the baculovirus protein expression system. 2012-07 Thesis http://eprints.usm.my/44880/ http://eprints.usm.my/44880/1/OOI%20LI%20CHING.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1 Natural history (General - Including nature conservation
geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation
geographical distribution)
Ooi, Li Ching
Screening For Small Molecule Inhibitors Of p27Kip1 Degradation
description In a majority of human cancers, the cyclin-dependent kinase (CDK) inhibitor p27Kip1 is commonly found to be deregulated due to proteolysis by the ubiquitin-proteasome pathway involving the SCFSkp2 E3 ligase. Although proteasome inhibitors act to stabilize p27Kip1, small molecules agents inhibiting the degradation of p27Kip1 by the function of SCFSkp2 E3 ligase may contribute to the development of a specific targeted drug that could prove to be more efficacious with less side effects. In this work, we have developed a high-throughput screening system based on phosphorylation dependent protein-protein interaction using the baculovirus protein expression system.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Ooi, Li Ching
author_facet Ooi, Li Ching
author_sort Ooi, Li Ching
title Screening For Small Molecule Inhibitors Of p27Kip1 Degradation
title_short Screening For Small Molecule Inhibitors Of p27Kip1 Degradation
title_full Screening For Small Molecule Inhibitors Of p27Kip1 Degradation
title_fullStr Screening For Small Molecule Inhibitors Of p27Kip1 Degradation
title_full_unstemmed Screening For Small Molecule Inhibitors Of p27Kip1 Degradation
title_sort screening for small molecule inhibitors of p27kip1 degradation
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2012
url http://eprints.usm.my/44880/1/OOI%20LI%20CHING.pdf
_version_ 1747821418256531456

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