Screening For Small Molecule Inhibitors Of p27Kip1 Degradation
In a majority of human cancers, the cyclin-dependent kinase (CDK) inhibitor p27Kip1 is commonly found to be deregulated due to proteolysis by the ubiquitin-proteasome pathway involving the SCFSkp2 E3 ligase. Although proteasome inhibitors act to stabilize p27Kip1, small molecules agents inhibiting t...
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2012
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my-usm-ep.448802019-07-05T01:56:11Z Screening For Small Molecule Inhibitors Of p27Kip1 Degradation 2012-07 Ooi, Li Ching QH1 Natural history (General - Including nature conservation, geographical distribution) In a majority of human cancers, the cyclin-dependent kinase (CDK) inhibitor p27Kip1 is commonly found to be deregulated due to proteolysis by the ubiquitin-proteasome pathway involving the SCFSkp2 E3 ligase. Although proteasome inhibitors act to stabilize p27Kip1, small molecules agents inhibiting the degradation of p27Kip1 by the function of SCFSkp2 E3 ligase may contribute to the development of a specific targeted drug that could prove to be more efficacious with less side effects. In this work, we have developed a high-throughput screening system based on phosphorylation dependent protein-protein interaction using the baculovirus protein expression system. 2012-07 Thesis http://eprints.usm.my/44880/ http://eprints.usm.my/44880/1/OOI%20LI%20CHING.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
| spellingShingle |
QH1 Natural history (General - Including nature conservation geographical distribution) Ooi, Li Ching Screening For Small Molecule Inhibitors Of p27Kip1 Degradation |
| description |
In a majority of human cancers, the cyclin-dependent kinase (CDK) inhibitor p27Kip1 is commonly found to be deregulated due to proteolysis by the ubiquitin-proteasome pathway involving the SCFSkp2 E3 ligase. Although proteasome inhibitors act to stabilize p27Kip1, small molecules agents inhibiting the degradation of p27Kip1 by the function of SCFSkp2 E3 ligase may contribute to the development of a specific targeted drug that could prove to be more efficacious with less side effects. In this work, we have developed a high-throughput screening system based on phosphorylation dependent protein-protein interaction using the baculovirus protein expression system. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Ooi, Li Ching |
| author_facet |
Ooi, Li Ching |
| author_sort |
Ooi, Li Ching |
| title |
Screening For Small Molecule Inhibitors Of p27Kip1 Degradation |
| title_short |
Screening For Small Molecule Inhibitors Of p27Kip1 Degradation |
| title_full |
Screening For Small Molecule Inhibitors Of p27Kip1 Degradation |
| title_fullStr |
Screening For Small Molecule Inhibitors Of p27Kip1 Degradation |
| title_full_unstemmed |
Screening For Small Molecule Inhibitors Of p27Kip1 Degradation |
| title_sort |
screening for small molecule inhibitors of p27kip1 degradation |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat |
| publishDate |
2012 |
| url |
http://eprints.usm.my/44880/1/OOI%20LI%20CHING.pdf |
| _version_ |
1747821418256531456 |