Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids
Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be in...
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| Format: | Thesis |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.usm.my/44885/1/RAFIDAH%20ISHAK.pdf |
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| Summary: | Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be induced from the leaf, petiole and root explants of the in vitro plantlets on MS medium supplemented with 2.0 - 10.0 mg/L picloram. The leaf explants inoculated to MS medium supplemented with 2.0 mg/L picloram produced the best callus for cell suspension cultures. |
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