Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids

Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be in...

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Main Author:	Ishak, Rafidah
Format:	Thesis
Language:	English
Published:	2012
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/44885/1/RAFIDAH%20ISHAK.pdf
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id	my-usm-ep.44885
record_format	uketd_dc
spelling	my-usm-ep.448852019-07-05T07:42:57Z Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids 2012-06 Ishak, Rafidah QH1 Natural history (General - Including nature conservation, geographical distribution) Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be induced from the leaf, petiole and root explants of the in vitro plantlets on MS medium supplemented with 2.0 - 10.0 mg/L picloram. The leaf explants inoculated to MS medium supplemented with 2.0 mg/L picloram produced the best callus for cell suspension cultures. 2012-06 Thesis http://eprints.usm.my/44885/ http://eprints.usm.my/44885/1/RAFIDAH%20ISHAK.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Ishak, Rafidah Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids
description	Aseptic seeds of Hyoscyamus niger were established via two-stage surface sterilization protocol using Clorox® solution coupled with Teepol with 95.6% of the seeds were aseptic. From these aseptic seeds, 79.8% of the seeds germinated and 100% of the seed-derived plantlets survived. Callus could be induced from the leaf, petiole and root explants of the in vitro plantlets on MS medium supplemented with 2.0 - 10.0 mg/L picloram. The leaf explants inoculated to MS medium supplemented with 2.0 mg/L picloram produced the best callus for cell suspension cultures.
format	Thesis
qualification_level	Master's degree
author	Ishak, Rafidah
author_facet	Ishak, Rafidah
author_sort	Ishak, Rafidah
title	Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids
title_short	Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids
title_full	Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids
title_fullStr	Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids
title_full_unstemmed	Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids
title_sort	establishment of cell suspension culture of hyoscyamus niger l. for the production of tropane alkaloids
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2012
url	http://eprints.usm.my/44885/1/RAFIDAH%20ISHAK.pdf
_version_	1747821419471831040

Establishment Of Cell Suspension Culture Of Hyoscyamus Niger L. For The Production Of Tropane Alkaloids

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