Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes

This work describes the effect of pH, temperature, incubation time, and substrate and enzyme weights on the hydrolysis of refined palm oil using the commercial lipase from the yeast Candida rugosa. It was found that the hydrolysis was optimum at pH 7.5, temperature 37 °C, incubation time 60 min and...

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Main Author: Jarjes, Zahraa A.
Format: Thesis
Language:English
Published: 2012
Subjects:
Online Access:http://eprints.usm.my/44930/1/ZAHRAA%20A.%20JARJES.pdf
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spelling my-usm-ep.449302019-07-09T07:07:57Z Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes 2012-07 Jarjes, Zahraa A. QD1-999 Chemistry This work describes the effect of pH, temperature, incubation time, and substrate and enzyme weights on the hydrolysis of refined palm oil using the commercial lipase from the yeast Candida rugosa. It was found that the hydrolysis was optimum at pH 7.5, temperature 37 °C, incubation time 60 min and the enzyme and substrate weights of 0.1 and 2 g, respectively. The released fatty acids were used as a substrate for lipoxygenase immobilized on modified Nafion membrane carbon electrode and the current generated was studied by cyclic voltammetry. Potassium buffer at pH 7 and 0.4 mg of lipoxygenase was found to be crucial in order to produce a higher current density. 2012-07 Thesis http://eprints.usm.my/44930/ http://eprints.usm.my/44930/1/ZAHRAA%20A.%20JARJES.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kimia
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QD1-999 Chemistry
spellingShingle QD1-999 Chemistry
Jarjes, Zahraa A.
Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
description This work describes the effect of pH, temperature, incubation time, and substrate and enzyme weights on the hydrolysis of refined palm oil using the commercial lipase from the yeast Candida rugosa. It was found that the hydrolysis was optimum at pH 7.5, temperature 37 °C, incubation time 60 min and the enzyme and substrate weights of 0.1 and 2 g, respectively. The released fatty acids were used as a substrate for lipoxygenase immobilized on modified Nafion membrane carbon electrode and the current generated was studied by cyclic voltammetry. Potassium buffer at pH 7 and 0.4 mg of lipoxygenase was found to be crucial in order to produce a higher current density.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Jarjes, Zahraa A.
author_facet Jarjes, Zahraa A.
author_sort Jarjes, Zahraa A.
title Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_short Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_full Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_fullStr Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_full_unstemmed Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_sort electrochemical studies of hydrolytic products from refined palm oil with immobilized enzymes at modified carbon electrodes
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kimia
publishDate 2012
url http://eprints.usm.my/44930/1/ZAHRAA%20A.%20JARJES.pdf
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