Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes

This work describes the effect of pH, temperature, incubation time, and substrate and enzyme weights on the hydrolysis of refined palm oil using the commercial lipase from the yeast Candida rugosa. It was found that the hydrolysis was optimum at pH 7.5, temperature 37 °C, incubation time 60 min and...

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Main Author:	Jarjes, Zahraa A.
Format:	Thesis
Language:	English
Published:	2012
Subjects:	QD1-999 Chemistry
Online Access:	http://eprints.usm.my/44930/1/ZAHRAA%20A.%20JARJES.pdf
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id	my-usm-ep.44930
record_format	uketd_dc
spelling	my-usm-ep.449302019-07-09T07:07:57Z Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes 2012-07 Jarjes, Zahraa A. QD1-999 Chemistry This work describes the effect of pH, temperature, incubation time, and substrate and enzyme weights on the hydrolysis of refined palm oil using the commercial lipase from the yeast Candida rugosa. It was found that the hydrolysis was optimum at pH 7.5, temperature 37 °C, incubation time 60 min and the enzyme and substrate weights of 0.1 and 2 g, respectively. The released fatty acids were used as a substrate for lipoxygenase immobilized on modified Nafion membrane carbon electrode and the current generated was studied by cyclic voltammetry. Potassium buffer at pH 7 and 0.4 mg of lipoxygenase was found to be crucial in order to produce a higher current density. 2012-07 Thesis http://eprints.usm.my/44930/ http://eprints.usm.my/44930/1/ZAHRAA%20A.%20JARJES.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QD1-999 Chemistry
spellingShingle	QD1-999 Chemistry Jarjes, Zahraa A. Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
description	This work describes the effect of pH, temperature, incubation time, and substrate and enzyme weights on the hydrolysis of refined palm oil using the commercial lipase from the yeast Candida rugosa. It was found that the hydrolysis was optimum at pH 7.5, temperature 37 °C, incubation time 60 min and the enzyme and substrate weights of 0.1 and 2 g, respectively. The released fatty acids were used as a substrate for lipoxygenase immobilized on modified Nafion membrane carbon electrode and the current generated was studied by cyclic voltammetry. Potassium buffer at pH 7 and 0.4 mg of lipoxygenase was found to be crucial in order to produce a higher current density.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Jarjes, Zahraa A.
author_facet	Jarjes, Zahraa A.
author_sort	Jarjes, Zahraa A.
title	Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_short	Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_full	Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_fullStr	Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_full_unstemmed	Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes
title_sort	electrochemical studies of hydrolytic products from refined palm oil with immobilized enzymes at modified carbon electrodes
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kimia
publishDate	2012
url	http://eprints.usm.my/44930/1/ZAHRAA%20A.%20JARJES.pdf
_version_	1747821427500777472

Electrochemical Studies Of Hydrolytic Products From Refined Palm Oil With Immobilized Enzymes At Modified Carbon Electrodes

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