Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium

Introduction: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient time. Antifungal activity is amongst the medicinal properties owned by Aloe vera. This study proposes antifungal effect of Malaysian Aloe vera leaf extract on pathogenic otomycosis species, Asp...

Full description

Saved in:

Bibliographic Details
Main Author:	Saniasiaya, Jeyasakthy
Format:	Thesis
Language:	English
Published:	2017
Subjects:	RB Pathology
Online Access:	http://eprints.usm.my/44982/1/Dr.%20Jeyasakthy%20Saniasiya-24%20pages.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.44982
record_format	uketd_dc
spelling	my-usm-ep.449822020-10-22T03:03:15Z Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium 2017 Saniasiaya, Jeyasakthy RB Pathology Introduction: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient time. Antifungal activity is amongst the medicinal properties owned by Aloe vera. This study proposes antifungal effect of Malaysian Aloe vera leaf extract on pathogenic otomycosis species, Aspergillus niger and Candida albicans. Methodology: This pilot study is a laboratory-controlled prospective study conducted in Universiti Sains Malaysia. Powdered form of Malaysian Aloe vera leaf was extracted with 70% ethanol and aqueous via Soxhlet extraction method, after being evaporated using rotary evaporator to a thicker compound. The concentrated extract is then freeze dried to obtain powdered form which is diluted into establish five different concentrations of 50 g/ml, 25 g/ml, 12.5 g/ml, 6.25 g/ml and 3.125 g/ml. Five replicates were performed for five different concentration. Results were statistically analysed using one-way ANOVA and independant-t test. Sabaroud Dextrose Agar lawned with tested fungal isolates were inoculated with the extracts using well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration. Results: For Aspergillus niger, zone of inhibition for alcohol and aqueous extract were obtained for all concentrations except 3.125g/ml. As for Candida albicans, there were no zone of inhibition for both alcohol and aqueous extract of Aloe vera leaf. The minimum inhibitory concentration value of aqueous and alcohol extracts were 5.129g/ml and 4.35g/ml for Aspergillus niger and as for Candida albicans, it was not determined as no zone of inhibition was obtained. 2017 Thesis http://eprints.usm.my/44982/ http://eprints.usm.my/44982/1/Dr.%20Jeyasakthy%20Saniasiya-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RB Pathology
spellingShingle	RB Pathology Saniasiaya, Jeyasakthy Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
description	Introduction: Aloe barbadensis miller or Aloe vera has been used for therapeutic purposes since ancient time. Antifungal activity is amongst the medicinal properties owned by Aloe vera. This study proposes antifungal effect of Malaysian Aloe vera leaf extract on pathogenic otomycosis species, Aspergillus niger and Candida albicans. Methodology: This pilot study is a laboratory-controlled prospective study conducted in Universiti Sains Malaysia. Powdered form of Malaysian Aloe vera leaf was extracted with 70% ethanol and aqueous via Soxhlet extraction method, after being evaporated using rotary evaporator to a thicker compound. The concentrated extract is then freeze dried to obtain powdered form which is diluted into establish five different concentrations of 50 g/ml, 25 g/ml, 12.5 g/ml, 6.25 g/ml and 3.125 g/ml. Five replicates were performed for five different concentration. Results were statistically analysed using one-way ANOVA and independant-t test. Sabaroud Dextrose Agar lawned with tested fungal isolates were inoculated with the extracts using well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration. Results: For Aspergillus niger, zone of inhibition for alcohol and aqueous extract were obtained for all concentrations except 3.125g/ml. As for Candida albicans, there were no zone of inhibition for both alcohol and aqueous extract of Aloe vera leaf. The minimum inhibitory concentration value of aqueous and alcohol extracts were 5.129g/ml and 4.35g/ml for Aspergillus niger and as for Candida albicans, it was not determined as no zone of inhibition was obtained.
format	Thesis
qualification_level	Master's degree
author	Saniasiaya, Jeyasakthy
author_facet	Saniasiaya, Jeyasakthy
author_sort	Saniasiaya, Jeyasakthy
title	Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_short	Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_full	Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_fullStr	Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_full_unstemmed	Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
title_sort	antifungal effect of malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2017
url	http://eprints.usm.my/44982/1/Dr.%20Jeyasakthy%20Saniasiya-24%20pages.pdf
_version_	1747821432638799872

Antifungal effect of Malaysian aloe vera leaf extract selected fungal species of pathologenic otomycosis in in-vitro culture medium

Similar Items