Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology

Systematic Evolution of Ligands by Exponential Enrichment (SELEX) enables the isolation of aptamer (ssDNA or RNA that possesses high binding affinity and specificity against virtually any target molecules). In this study, a RNA aptamer against recombinant human EPO-alpha (rHuEPO-α) was successfully...

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Main Author:	Marimuthu, Citartan
Format:	Thesis
Language:	English
Published:	2014
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/44994/1/CITARTAN%20MARIMUTHU.pdf
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spelling	my-usm-ep.449942019-07-22T03:03:01Z Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology 2014-03 Marimuthu, Citartan R735-854 Medical education. Medical schools. Research Systematic Evolution of Ligands by Exponential Enrichment (SELEX) enables the isolation of aptamer (ssDNA or RNA that possesses high binding affinity and specificity against virtually any target molecules). In this study, a RNA aptamer against recombinant human EPO-alpha (rHuEPO-α) was successfully isolated by SELEX technology. After 11 cycles of SELEX, cloning and sequence analysis showed an appearance of a single major clone constituting the putative RNA aptamer, termed REPORA-6, with a dissociation constant of 25±1 nM. 2014-03 Thesis http://eprints.usm.my/44994/ http://eprints.usm.my/44994/1/CITARTAN%20MARIMUTHU.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Marimuthu, Citartan Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology
description	Systematic Evolution of Ligands by Exponential Enrichment (SELEX) enables the isolation of aptamer (ssDNA or RNA that possesses high binding affinity and specificity against virtually any target molecules). In this study, a RNA aptamer against recombinant human EPO-alpha (rHuEPO-α) was successfully isolated by SELEX technology. After 11 cycles of SELEX, cloning and sequence analysis showed an appearance of a single major clone constituting the putative RNA aptamer, termed REPORA-6, with a dissociation constant of 25±1 nM.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Marimuthu, Citartan
author_facet	Marimuthu, Citartan
author_sort	Marimuthu, Citartan
title	Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology
title_short	Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology
title_full	Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology
title_fullStr	Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology
title_full_unstemmed	Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology
title_sort	generation and characterization of rna aptamer against rhuepo-a by selex technology
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2014
url	http://eprints.usm.my/44994/1/CITARTAN%20MARIMUTHU.pdf
_version_	1747821434339590144

Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology

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