A Novel Nitrocellulose Filter Membrane Based Partitioning-Reverse Transcription Using Repora-6 RNA Aptamer In Detecting Erythropoietin (EPO)
Recombinant human EPO (rHuEPO), a mimetic of endogenous EPO is widely misused by athletes as a performance enhancer, a phenomenon known as doping. Current methods of detecting rHuEPO doping are via isoelectric-focusing (IEF)-doubleimmunoblotting, Sarcosyl-PAGE (SAR PAGE) and lateral flow test, which...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://eprints.usm.my/45040/1/PRESELA%20RAVINDERAN.pdf |
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| Summary: | Recombinant human EPO (rHuEPO), a mimetic of endogenous EPO is widely misused by athletes as a performance enhancer, a phenomenon known as doping. Current methods of detecting rHuEPO doping are via isoelectric-focusing (IEF)-doubleimmunoblotting, Sarcosyl-PAGE (SAR PAGE) and lateral flow test, which are antibody-based approach using anti-EPO antibody. The caveats associated with anti- EPO antibodies have spurred the interest to adopt an alternative molecular recognition element (MRE) in detecting EPO. |
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