Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model

Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model

2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant type of carcinogenic heterocyclic amine produced in cooked meat. This study aims to investigate the carcinogenic effect of human physiologically relevant concentration of PhIP on human prostate epithelial cell line, RWPE-1,...

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Main Author: Saifuddin, Siti Nazmin
Format: Thesis
Language:English
Published: 2019
Subjects:
R735-854 Medical education
Medical schools
Research
Online Access:http://eprints.usm.my/45071/1/SITI%20NAZMIN%20SAIFUDDIN.pdf
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spelling my-usm-ep.450712019-07-26T01:08:51Z Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model 2019-01 Saifuddin, Siti Nazmin R735-854 Medical education. Medical schools. Research 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant type of carcinogenic heterocyclic amine produced in cooked meat. This study aims to investigate the carcinogenic effect of human physiologically relevant concentration of PhIP on human prostate epithelial cell line, RWPE-1, at cellular and protein levels in prostate carcinogenesis. RWPE-1 cells were exposed to 10-7, 10-8, 10-9 or 10-10 M PhIP, diluted in 0.003%, 0.025% or 0.25% DMSO, with or without metabolic activation system. The methodologies used in this study were MTS assay for cytotoxicity and cell proliferation tests, Alamar Blue assay for cell proliferation test, cell transformation assay, invasion assay and shotgun proteomic. 2019-01 Thesis http://eprints.usm.my/45071/ http://eprints.usm.my/45071/1/SITI%20NAZMIN%20SAIFUDDIN.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R735-854 Medical education
Medical schools
Research
spellingShingle R735-854 Medical education
Medical schools
Research
Saifuddin, Siti Nazmin
Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model
description 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant type of carcinogenic heterocyclic amine produced in cooked meat. This study aims to investigate the carcinogenic effect of human physiologically relevant concentration of PhIP on human prostate epithelial cell line, RWPE-1, at cellular and protein levels in prostate carcinogenesis. RWPE-1 cells were exposed to 10-7, 10-8, 10-9 or 10-10 M PhIP, diluted in 0.003%, 0.025% or 0.25% DMSO, with or without metabolic activation system. The methodologies used in this study were MTS assay for cytotoxicity and cell proliferation tests, Alamar Blue assay for cell proliferation test, cell transformation assay, invasion assay and shotgun proteomic.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Saifuddin, Siti Nazmin
author_facet Saifuddin, Siti Nazmin
author_sort Saifuddin, Siti Nazmin
title Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model
title_short Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model
title_full Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model
title_fullStr Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model
title_full_unstemmed Proteomic Analysis Of Prostate Carcinogenesis Induced By Heterocyclic Amine 2-Amino-1-Methyl- 6-Phenylimidazo[4,5-B]Pyridine (PhIP) Using In Vitro Model
title_sort proteomic analysis of prostate carcinogenesis induced by heterocyclic amine 2-amino-1-methyl- 6-phenylimidazo[4,5-b]pyridine (phip) using in vitro model
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2019
url http://eprints.usm.my/45071/1/SITI%20NAZMIN%20SAIFUDDIN.pdf
_version_ 1747821450196156416

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