Antiproliferative Properties Of Clitoria Ternatea And Vitex Negundo On Cancer Cell Lines

Leaves and flowers of Clitoria ternatea and Vitex negundo were evaluated by proximate analysis using the AOAC method. The mineral and heavy metal contents in both plants were determined using the Inductively Coupled Plasma Optical Emission Spectrophotometer (ICP-OES). The major aim of this study was...

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Main Author: Dashti, Neda Ganjali
Format: Thesis
Language:English
Published: 2013
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Online Access:http://eprints.usm.my/45161/1/Neda%20Ganjali%20Dashti24.pdf
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spelling my-usm-ep.451612019-08-02T07:41:06Z Antiproliferative Properties Of Clitoria Ternatea And Vitex Negundo On Cancer Cell Lines 2013-07 Dashti, Neda Ganjali T11.95-12.5 Industrial directories Leaves and flowers of Clitoria ternatea and Vitex negundo were evaluated by proximate analysis using the AOAC method. The mineral and heavy metal contents in both plants were determined using the Inductively Coupled Plasma Optical Emission Spectrophotometer (ICP-OES). The major aim of this study was to examine the anti-proliferative activities (IC50 value <100 μg/mL) of water and methanol extracts of both plants against human cancer origin cell lines, namely MCF-7 (hormone-dependent breast cancer cell line), MDA-MB-231 (non-hormone-dependent breast cancer cell line), Caov-3 (human ovary cancer cell line), Hela (human cervical cancer cell line), HepG2 (human liver cancer cell line) and Hs27 (human non-cancer-origin fibroblast cell line). The cell growth inhibition was evaluated by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay for 24, 48 and 72 hours. Results from the proximate analysis indicated that the leaves and flowers of Vitex negundo and Clitoria ternatea were composed of fiber (2.1% to 11.6%), carbohydrate (2.2% to 18.9%), protein (0.32 to 0.87%), fat (2.5% to 7%), ash (0.45% to 8%) and moisture (53.4% to 92.4%), respectively. Leaves and flowers of Vitex negundo were rich in calcium (10.5 and 5.5 mg/g), respectively. All the analysis of heavy metals (Ni, Cd, As, Pb) were found to be at a lower concentration and were below the detection limits, thus making the plants safer for consumption. 2013-07 Thesis http://eprints.usm.my/45161/ http://eprints.usm.my/45161/1/Neda%20Ganjali%20Dashti24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic T11.95-12.5 Industrial directories
spellingShingle T11.95-12.5 Industrial directories
Dashti, Neda Ganjali
Antiproliferative Properties Of Clitoria Ternatea And Vitex Negundo On Cancer Cell Lines
description Leaves and flowers of Clitoria ternatea and Vitex negundo were evaluated by proximate analysis using the AOAC method. The mineral and heavy metal contents in both plants were determined using the Inductively Coupled Plasma Optical Emission Spectrophotometer (ICP-OES). The major aim of this study was to examine the anti-proliferative activities (IC50 value <100 μg/mL) of water and methanol extracts of both plants against human cancer origin cell lines, namely MCF-7 (hormone-dependent breast cancer cell line), MDA-MB-231 (non-hormone-dependent breast cancer cell line), Caov-3 (human ovary cancer cell line), Hela (human cervical cancer cell line), HepG2 (human liver cancer cell line) and Hs27 (human non-cancer-origin fibroblast cell line). The cell growth inhibition was evaluated by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay for 24, 48 and 72 hours. Results from the proximate analysis indicated that the leaves and flowers of Vitex negundo and Clitoria ternatea were composed of fiber (2.1% to 11.6%), carbohydrate (2.2% to 18.9%), protein (0.32 to 0.87%), fat (2.5% to 7%), ash (0.45% to 8%) and moisture (53.4% to 92.4%), respectively. Leaves and flowers of Vitex negundo were rich in calcium (10.5 and 5.5 mg/g), respectively. All the analysis of heavy metals (Ni, Cd, As, Pb) were found to be at a lower concentration and were below the detection limits, thus making the plants safer for consumption.
format Thesis
qualification_level Master's degree
author Dashti, Neda Ganjali
author_facet Dashti, Neda Ganjali
author_sort Dashti, Neda Ganjali
title Antiproliferative Properties Of Clitoria Ternatea And Vitex Negundo On Cancer Cell Lines
title_short Antiproliferative Properties Of Clitoria Ternatea And Vitex Negundo On Cancer Cell Lines
title_full Antiproliferative Properties Of Clitoria Ternatea And Vitex Negundo On Cancer Cell Lines
title_fullStr Antiproliferative Properties Of Clitoria Ternatea And Vitex Negundo On Cancer Cell Lines
title_full_unstemmed Antiproliferative Properties Of Clitoria Ternatea And Vitex Negundo On Cancer Cell Lines
title_sort antiproliferative properties of clitoria ternatea and vitex negundo on cancer cell lines
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Teknologi Industri
publishDate 2013
url http://eprints.usm.my/45161/1/Neda%20Ganjali%20Dashti24.pdf
_version_ 1747821460519387136