Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes

Six ternary copper(II) complexes of amino acids and polypyridyl ligands with a general formula of [Cu(AA)(B) Cl]·nH2O or [Cu(AA)(B)(H2O)]Cl·nH2O, where AA = DL-threonine, DL-phenylalanine, and DL-valine; B = 2,2’-bipyridyl, 4,4’-dimethyl-2,2’-dipyridyl, and 1,10-phenanthroline are synthesized. The c...

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Main Author:	Tan, Yi Han
Format:	Thesis
Language:	English
Published:	2013
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/45216/1/Tan%20Yi%20Han24.pdf
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spelling	my-usm-ep.452162019-08-07T06:50:45Z Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes 2013-07 Tan, Yi Han TP1-1185 Chemical technology Six ternary copper(II) complexes of amino acids and polypyridyl ligands with a general formula of [Cu(AA)(B) Cl]·nH2O or [Cu(AA)(B)(H2O)]Cl·nH2O, where AA = DL-threonine, DL-phenylalanine, and DL-valine; B = 2,2’-bipyridyl, 4,4’-dimethyl-2,2’-dipyridyl, and 1,10-phenanthroline are synthesized. The complexes are characterized by CHN elemental analysis, AAS, FT-IR, and UV-Vis and have been structurally characterized by X-ray crystallography. All the complexes formed slightly distorted square-pyramidal coordination geometries. The electronic absorption spectra of the complexes show very low intensity of d-d electronic band in the range of 600-635 nm in Tris-HCl/NaCl (5:5 mM) pH 7.2 buffer solution. The DNA binding interaction with CT-DNA has been investigated by electronic absorption spectral titration and viscosity measurements. The results revealed that the phenanthroline complexes interact with CT-DNA through intercalation while bipyridyl complexes bind to CT-DNA through groove binding mode. The calculated intrinsic binding constant (Kb) of all the complexes are in the range of 0.5-6.2 × 105 M-1. All the copper(II) complexes are found to promote efficient DNA cleavage activities at low concentration in the presence of H2O2. The studies show that [Cu(Phe)(Phen)Cl]·3H2O exhibits the highest DNA binding and nuclease activity among the six copper(II) complexes. 2013-07 Thesis http://eprints.usm.my/45216/ http://eprints.usm.my/45216/1/Tan%20Yi%20Han24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Tan, Yi Han Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes
description	Six ternary copper(II) complexes of amino acids and polypyridyl ligands with a general formula of [Cu(AA)(B) Cl]·nH2O or [Cu(AA)(B)(H2O)]Cl·nH2O, where AA = DL-threonine, DL-phenylalanine, and DL-valine; B = 2,2’-bipyridyl, 4,4’-dimethyl-2,2’-dipyridyl, and 1,10-phenanthroline are synthesized. The complexes are characterized by CHN elemental analysis, AAS, FT-IR, and UV-Vis and have been structurally characterized by X-ray crystallography. All the complexes formed slightly distorted square-pyramidal coordination geometries. The electronic absorption spectra of the complexes show very low intensity of d-d electronic band in the range of 600-635 nm in Tris-HCl/NaCl (5:5 mM) pH 7.2 buffer solution. The DNA binding interaction with CT-DNA has been investigated by electronic absorption spectral titration and viscosity measurements. The results revealed that the phenanthroline complexes interact with CT-DNA through intercalation while bipyridyl complexes bind to CT-DNA through groove binding mode. The calculated intrinsic binding constant (Kb) of all the complexes are in the range of 0.5-6.2 × 105 M-1. All the copper(II) complexes are found to promote efficient DNA cleavage activities at low concentration in the presence of H2O2. The studies show that [Cu(Phe)(Phen)Cl]·3H2O exhibits the highest DNA binding and nuclease activity among the six copper(II) complexes.
format	Thesis
qualification_level	Master's degree
author	Tan, Yi Han
author_facet	Tan, Yi Han
author_sort	Tan, Yi Han
title	Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes
title_short	Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes
title_full	Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes
title_fullStr	Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes
title_full_unstemmed	Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes
title_sort	synthesis, characterization, oxidative dna cleavage and dna binding studies of mixed ligands copper(ii) complexes
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2013
url	http://eprints.usm.my/45216/1/Tan%20Yi%20Han24.pdf
_version_	1747821470772363264

Synthesis, Characterization, Oxidative Dna Cleavage And Dna Binding Studies Of Mixed Ligands Copper(Ii) Complexes

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