Expression of the transcription factor, PP ARs in immune cells of balb/c mice

Peroxisome proliferator activated receptors (PP ARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. Despite significant involvement in the lipid metabolism, little is known about the expression pattern and the role of different PP ARs in immune...

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Main Author:	Letchumanan, Mobana
Format:	Thesis
Language:	English
Published:	2004
Subjects:	RJ Pediatrics
Online Access:	http://eprints.usm.my/45244/1/LI...Mohana%20Letchumanan...2004...-24%20pages.pdf
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id	my-usm-ep.45244
record_format	uketd_dc
spelling	my-usm-ep.452442019-08-15T08:39:34Z Expression of the transcription factor, PP ARs in immune cells of balb/c mice 2004 Letchumanan, Mobana RJ Pediatrics Peroxisome proliferator activated receptors (PP ARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. Despite significant involvement in the lipid metabolism, little is known about the expression pattern and the role of different PP ARs in immune regulation. Emerging evidence suggests that PP ARs may act as negative immunomodulators. To provide further insight into the role ofPPARs in the immune system, the expression patterns of PPARa., PPARyl and PPARy2 in peritoneal macrophages and splenocyte-derived T lymphocyte subsets, CD4+ and CD8+ cells of balb/c mice were studied. Total RNA from each of the cell subsets was extracted, reverse-transcribed to eDNA and the expression of PPARa, PPARyl and PPARy2 quantified by Real-Time PCR using specific primers and probes. The expression levels were then calculated per J..lg total RNA and statistically analyzed using statistical software. In addition, cytokine expression profiles in the selected immune cells were also assessed using multiplex PCR method. The results obtained in the present study demonstrate that all the immune cell subtypes predominantly express PP ARyl suggesting the probable involvement of this transcription factor in immune response or other biological functions of these cells. The study also further confirms that lymphocytes express PP ARa. CD8+ lymphocytes showed the lowest expression of all PP AR isoforms compared to other immune cells suggesting minimal involvement of the PP ARs in the immune response mediated by these cells. In addition baseline expression levels of various cytokines in immune cell subsets in young adult mice also were showed in the current study. 2004 Thesis http://eprints.usm.my/45244/ http://eprints.usm.my/45244/1/LI...Mohana%20Letchumanan...2004...-24%20pages.pdf application/pdf en public other Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RJ Pediatrics
spellingShingle	RJ Pediatrics Letchumanan, Mobana Expression of the transcription factor, PP ARs in immune cells of balb/c mice
description	Peroxisome proliferator activated receptors (PP ARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. Despite significant involvement in the lipid metabolism, little is known about the expression pattern and the role of different PP ARs in immune regulation. Emerging evidence suggests that PP ARs may act as negative immunomodulators. To provide further insight into the role ofPPARs in the immune system, the expression patterns of PPARa., PPARyl and PPARy2 in peritoneal macrophages and splenocyte-derived T lymphocyte subsets, CD4+ and CD8+ cells of balb/c mice were studied. Total RNA from each of the cell subsets was extracted, reverse-transcribed to eDNA and the expression of PPARa, PPARyl and PPARy2 quantified by Real-Time PCR using specific primers and probes. The expression levels were then calculated per J..lg total RNA and statistically analyzed using statistical software. In addition, cytokine expression profiles in the selected immune cells were also assessed using multiplex PCR method. The results obtained in the present study demonstrate that all the immune cell subtypes predominantly express PP ARyl suggesting the probable involvement of this transcription factor in immune response or other biological functions of these cells. The study also further confirms that lymphocytes express PP ARa. CD8+ lymphocytes showed the lowest expression of all PP AR isoforms compared to other immune cells suggesting minimal involvement of the PP ARs in the immune response mediated by these cells. In addition baseline expression levels of various cytokines in immune cell subsets in young adult mice also were showed in the current study.
format	Thesis
qualification_level	other
author	Letchumanan, Mobana
author_facet	Letchumanan, Mobana
author_sort	Letchumanan, Mobana
title	Expression of the transcription factor, PP ARs in immune cells of balb/c mice
title_short	Expression of the transcription factor, PP ARs in immune cells of balb/c mice
title_full	Expression of the transcription factor, PP ARs in immune cells of balb/c mice
title_fullStr	Expression of the transcription factor, PP ARs in immune cells of balb/c mice
title_full_unstemmed	Expression of the transcription factor, PP ARs in immune cells of balb/c mice
title_sort	expression of the transcription factor, pp ars in immune cells of balb/c mice
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2004
url	http://eprints.usm.my/45244/1/LI...Mohana%20Letchumanan...2004...-24%20pages.pdf
_version_	1747821475919822848

Expression of the transcription factor, PP ARs in immune cells of balb/c mice

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