The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency

Dengue virus is endemic in peninsular Malaysia. The clinical manifestations vary depending on the incubation period of the virus as well as the immunity of the patients. Glucose-6-phosphate dehydrogenase (G6PD) deficiency is prevalent in Malaysia, where the incidence is 5.2%. It has been noted that...

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Main Author:	Al-Alimi, Abdullah Abdull Jabbar Ahmed
Format:	Thesis
Language:	English
Published:	2013
Subjects:	R5-130.5 General works
Online Access:	http://eprints.usm.my/45253/1/Abdullah%20Abdull%20Jabbar%20Ahmed%20Al-Alimi24.pdf
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id	my-usm-ep.45253
record_format	uketd_dc
spelling	my-usm-ep.452532019-08-22T07:01:40Z The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency 2013-07 Al-Alimi, Abdullah Abdull Jabbar Ahmed R5-130.5 General works Dengue virus is endemic in peninsular Malaysia. The clinical manifestations vary depending on the incubation period of the virus as well as the immunity of the patients. Glucose-6-phosphate dehydrogenase (G6PD) deficiency is prevalent in Malaysia, where the incidence is 5.2%. It has been noted that G6PD-deficient individuals suffer from more severe clinical presentation of dengue infection. The aim of this study is to investigate the susceptibility of monocytes to DENV2 infection, the oxidative responses, and cytokine production in monocytes from G6PD-deficient individuals. Blood samples were collected from donors after being approved by the Research Ethical Committees of USM and Ministry of Health. Screening for G6PD was performed using the florescent spot method followed by the quantitative assay. Monocytes from G6PD-deficient and healthy individuals (G6PD-normal) were isolated and infected with DENV2, whereby multiplicity of infection (MOI) 0.1 and intracellular infection rate were measured by flow cytometry and extracellular by plaque assay. Levels of oxidative species, superoxide anions (O2•-), nitric oxide (NO), oxidative stress and cytokines were determined and compared with normal controls. 2013-07 Thesis http://eprints.usm.my/45253/ http://eprints.usm.my/45253/1/Abdullah%20Abdull%20Jabbar%20Ahmed%20Al-Alimi24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-130.5 General works
spellingShingle	R5-130.5 General works Al-Alimi, Abdullah Abdull Jabbar Ahmed The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency
description	Dengue virus is endemic in peninsular Malaysia. The clinical manifestations vary depending on the incubation period of the virus as well as the immunity of the patients. Glucose-6-phosphate dehydrogenase (G6PD) deficiency is prevalent in Malaysia, where the incidence is 5.2%. It has been noted that G6PD-deficient individuals suffer from more severe clinical presentation of dengue infection. The aim of this study is to investigate the susceptibility of monocytes to DENV2 infection, the oxidative responses, and cytokine production in monocytes from G6PD-deficient individuals. Blood samples were collected from donors after being approved by the Research Ethical Committees of USM and Ministry of Health. Screening for G6PD was performed using the florescent spot method followed by the quantitative assay. Monocytes from G6PD-deficient and healthy individuals (G6PD-normal) were isolated and infected with DENV2, whereby multiplicity of infection (MOI) 0.1 and intracellular infection rate were measured by flow cytometry and extracellular by plaque assay. Levels of oxidative species, superoxide anions (O2•-), nitric oxide (NO), oxidative stress and cytokines were determined and compared with normal controls.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Al-Alimi, Abdullah Abdull Jabbar Ahmed
author_facet	Al-Alimi, Abdullah Abdull Jabbar Ahmed
author_sort	Al-Alimi, Abdullah Abdull Jabbar Ahmed
title	The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency
title_short	The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency
title_full	The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency
title_fullStr	The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency
title_full_unstemmed	The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency
title_sort	study of susceptibility and cytokine production in dengue virus type 2 (denv2)-infected monocytes of individuals with glucose-6-phosphate dehydrogenase (g6pd) deficiency
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2013
url	http://eprints.usm.my/45253/1/Abdullah%20Abdull%20Jabbar%20Ahmed%20Al-Alimi24.pdf
_version_	1747821477381537792

The Study Of Susceptibility And Cytokine Production In Dengue Virus Type 2 (Denv2)-Infected Monocytes Of Individuals With Glucose-6-Phosphate Dehydrogenase (G6pd) Deficiency

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