Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation

In nature, chicken feathers were abundantly generated from poultry industry and become severe environmental problems. The capabilities of microbial degradation and bioconversion of chicken feathers were directed to the reason for this study. The present study used the keratinaceous waste as a substr...

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Main Author:	Alyas, Nur Diyana
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/45260/1/Nur%20Diyana%20Binti%20Alyas24.pdf
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id	my-usm-ep.45260
record_format	uketd_dc
spelling	my-usm-ep.452602019-08-22T08:30:36Z Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation 2013-07 Alyas, Nur Diyana QH1 Natural history (General - Including nature conservation, geographical distribution) In nature, chicken feathers were abundantly generated from poultry industry and become severe environmental problems. The capabilities of microbial degradation and bioconversion of chicken feathers were directed to the reason for this study. The present study used the keratinaceous waste as a substrate in keratinase production from molecular identification of M. fulvum IBRL SD3 via solid substrate fermentation. The improvements of cultural conditions for maximal keratinase production in a shake flask system with particles size of substrate of 0.75 mm, at the initial moisture content of 100% (w/w), cultivation temperature of room temperature (30±2ºC), initial pH 7, mixing frequency at once every 24 hours and inoculum size of 1 X 107 spores/ml showed keratinase achieved 0.266 U/g of fermented substrate on day 6th of cultivation. In the improvement of medium compositions, no additional carbon source was required and a slight supplementation of 0.70% (w/w) of yeast extract to produce the maximum keratinase activity on day 6th of cultivation at 0.372 U/g of fermented substrate with the increment of keratinase activity at 905.41% compared to the initial profile. Furthermore, an improvement of tray system was conducted using a shallow aluminium tray (16 cm x 16 cm x 5 cm). 2013-07 Thesis http://eprints.usm.my/45260/ http://eprints.usm.my/45260/1/Nur%20Diyana%20Binti%20Alyas24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Alyas, Nur Diyana Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
description	In nature, chicken feathers were abundantly generated from poultry industry and become severe environmental problems. The capabilities of microbial degradation and bioconversion of chicken feathers were directed to the reason for this study. The present study used the keratinaceous waste as a substrate in keratinase production from molecular identification of M. fulvum IBRL SD3 via solid substrate fermentation. The improvements of cultural conditions for maximal keratinase production in a shake flask system with particles size of substrate of 0.75 mm, at the initial moisture content of 100% (w/w), cultivation temperature of room temperature (30±2ºC), initial pH 7, mixing frequency at once every 24 hours and inoculum size of 1 X 107 spores/ml showed keratinase achieved 0.266 U/g of fermented substrate on day 6th of cultivation. In the improvement of medium compositions, no additional carbon source was required and a slight supplementation of 0.70% (w/w) of yeast extract to produce the maximum keratinase activity on day 6th of cultivation at 0.372 U/g of fermented substrate with the increment of keratinase activity at 905.41% compared to the initial profile. Furthermore, an improvement of tray system was conducted using a shallow aluminium tray (16 cm x 16 cm x 5 cm).
format	Thesis
qualification_level	Master's degree
author	Alyas, Nur Diyana
author_facet	Alyas, Nur Diyana
author_sort	Alyas, Nur Diyana
title	Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_short	Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_full	Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_fullStr	Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_full_unstemmed	Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation
title_sort	chicken feathers as an alternative substrate for extracellular keratinase production using microsporum fulvum ibrl sd3 by solid substrate fermentation
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2013
url	http://eprints.usm.my/45260/1/Nur%20Diyana%20Binti%20Alyas24.pdf
_version_	1747821479104348160

Chicken Feathers As An Alternative Substrate For Extracellular Keratinase Production Using Microsporum Fulvum Ibrl Sd3 By Solid Substrate Fermentation

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