Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate
Polyhydroxyalkanoate (PHA) is a family of polyesters produced by bacteria that possesses physical properties for various industrial applications. In this study, PHA synthase (PhaC) gene isolated from Chromobacterium sp. USM2 (phaCCs USM2) was explored for its potential to synthesize polymer in Cupri...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/45355/1/BALQIS%20AB.%20GHANI.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my-usm-ep.45355 |
|---|---|
| record_format |
uketd_dc |
| spelling |
my-usm-ep.453552019-09-10T02:45:45Z Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate 2017-05 Ab Ghani, Balqis QH1 Natural history (General - Including nature conservation, geographical distribution) Polyhydroxyalkanoate (PHA) is a family of polyesters produced by bacteria that possesses physical properties for various industrial applications. In this study, PHA synthase (PhaC) gene isolated from Chromobacterium sp. USM2 (phaCCs USM2) was explored for its potential to synthesize polymer in Cupriavidus necator. A broad-host range plasmid pBBR1MCS-2 was modified to construct an expression plasmid pYEB-100, which was designed to facilitate cloning of PHA synthases in order to assess the performance of various PhaCs and the composition of PHA accumulated in a standard environment. 2017-05 Thesis http://eprints.usm.my/45355/ http://eprints.usm.my/45355/1/BALQIS%20AB.%20GHANI.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
| spellingShingle |
QH1 Natural history (General - Including nature conservation geographical distribution) Ab Ghani, Balqis Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| description |
Polyhydroxyalkanoate (PHA) is a family of polyesters produced by bacteria that possesses physical properties for various industrial applications. In this study, PHA synthase (PhaC) gene isolated from Chromobacterium sp. USM2 (phaCCs USM2) was explored for its potential to synthesize polymer in Cupriavidus necator. A broad-host range plasmid pBBR1MCS-2 was modified to construct an expression plasmid pYEB-100, which was designed to facilitate cloning of PHA synthases in order to assess the performance of various PhaCs and the composition of PHA accumulated in a standard environment. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Ab Ghani, Balqis |
| author_facet |
Ab Ghani, Balqis |
| author_sort |
Ab Ghani, Balqis |
| title |
Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_short |
Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_full |
Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_fullStr |
Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_full_unstemmed |
Metabolic Engineering Of Cupriavidus Necator For The Production Of Poly (3-Hydroxybutyrate-Co-3-Hydroxyhexanoate |
| title_sort |
metabolic engineering of cupriavidus necator for the production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat |
| publishDate |
2017 |
| url |
http://eprints.usm.my/45355/1/BALQIS%20AB.%20GHANI.pdf |
| _version_ |
1747821492791410688 |