High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study

High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study

In order to accelerate the discovery of novel leads for the treatment of Human African Trypanosomiasis (HAT), it is necessary to have a simple and sensitive assay to identify positive hits by whole cell viability based high-throughput screening (HTS). In this study, the HTS assay was developed in 38...

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Main Author: Lim, Kah Tee
Format: Thesis
Language:English
Published: 2017
Subjects:
QH1-278.5 Natural history (General)
Online Access:http://eprints.usm.my/45404/1/LIM%20KAH%20TEE.pdf
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spelling my-usm-ep.454042019-09-12T02:08:57Z High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study 2017-02 Lim, Kah Tee QH1-278.5 Natural history (General) In order to accelerate the discovery of novel leads for the treatment of Human African Trypanosomiasis (HAT), it is necessary to have a simple and sensitive assay to identify positive hits by whole cell viability based high-throughput screening (HTS). In this study, the HTS assay was developed in 384-well format using clear plate and black plate, for comparison. Assay robustness and reproducibility were determined under the optimized conditions in 384-well plate was well tolerated in the HTS assay, including percentage of coefficient of variation of 4.68% and 4.74%, signal-to-background ratio of 12.75 and 12.07, and Z’ factor of 0.79 and 0.82 in clear- and black-384-well plate, respectively. 2017-02 Thesis http://eprints.usm.my/45404/ http://eprints.usm.my/45404/1/LIM%20KAH%20TEE.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Penyelidikan Biokimia Analisis
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Lim, Kah Tee
High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study
description In order to accelerate the discovery of novel leads for the treatment of Human African Trypanosomiasis (HAT), it is necessary to have a simple and sensitive assay to identify positive hits by whole cell viability based high-throughput screening (HTS). In this study, the HTS assay was developed in 384-well format using clear plate and black plate, for comparison. Assay robustness and reproducibility were determined under the optimized conditions in 384-well plate was well tolerated in the HTS assay, including percentage of coefficient of variation of 4.68% and 4.74%, signal-to-background ratio of 12.75 and 12.07, and Z’ factor of 0.79 and 0.82 in clear- and black-384-well plate, respectively.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Lim, Kah Tee
author_facet Lim, Kah Tee
author_sort Lim, Kah Tee
title High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study
title_short High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study
title_full High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study
title_fullStr High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study
title_full_unstemmed High-Throughput Screening Assay In 384-Well Format For The Identification Of Anti-Trypanosomal Agents Against Trypanosoma Brucei Rhodesiense And Mode Of Cell Death Study
title_sort high-throughput screening assay in 384-well format for the identification of anti-trypanosomal agents against trypanosoma brucei rhodesiense and mode of cell death study
granting_institution Universiti Sains Malaysia
granting_department Pusat Penyelidikan Biokimia Analisis
publishDate 2017
url http://eprints.usm.my/45404/1/LIM%20KAH%20TEE.pdf
_version_ 1747821503786778624

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