Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli

Escherichia coli is one of the most popular bacterial hosts for handling, manipulating, and expressing target genes of interest. Certain genes are difficult to express, often due to scarcity of specific tRNA. This has led to little and/or poor quality protein production in E. coli. To alleviate this...

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Main Author:	Che Nordin, Muhamad Alif
Format:	Thesis
Language:	English
Published:	2017
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/45424/1/MUHAMAD%20ALIF%20CHE%20NORDIN.pdf
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id	my-usm-ep.45424
record_format	uketd_dc
spelling	my-usm-ep.454242019-09-17T01:59:16Z Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli 2017-05 Che Nordin, Muhamad Alif R735-854 Medical education. Medical schools. Research Escherichia coli is one of the most popular bacterial hosts for handling, manipulating, and expressing target genes of interest. Certain genes are difficult to express, often due to scarcity of specific tRNA. This has led to little and/or poor quality protein production in E. coli. To alleviate this problem, E. coli hosts are transformed with rare tRNA-expressing helper plasmid vectors. Maintenance of two different plasmid vectors-expressing the gene of interest and rare tRNA genes exert metabolic burden on the host that may result in diminished growth and reduced protein production. 2017-05 Thesis http://eprints.usm.my/45424/ http://eprints.usm.my/45424/1/MUHAMAD%20ALIF%20CHE%20NORDIN.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Che Nordin, Muhamad Alif Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli
description	Escherichia coli is one of the most popular bacterial hosts for handling, manipulating, and expressing target genes of interest. Certain genes are difficult to express, often due to scarcity of specific tRNA. This has led to little and/or poor quality protein production in E. coli. To alleviate this problem, E. coli hosts are transformed with rare tRNA-expressing helper plasmid vectors. Maintenance of two different plasmid vectors-expressing the gene of interest and rare tRNA genes exert metabolic burden on the host that may result in diminished growth and reduced protein production.
format	Thesis
qualification_level	Master's degree
author	Che Nordin, Muhamad Alif
author_facet	Che Nordin, Muhamad Alif
author_sort	Che Nordin, Muhamad Alif
title	Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli
title_short	Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli
title_full	Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli
title_fullStr	Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli
title_full_unstemmed	Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli
title_sort	development of a novel t/a cloning and expression vector for efficient expression of protein-based biopharmaceuticals in escherichia coli
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2017
url	http://eprints.usm.my/45424/1/MUHAMAD%20ALIF%20CHE%20NORDIN.pdf
_version_	1747821508169826304

Development Of A Novel T/A Cloning And Expression Vector For Efficient Expression Of Protein-Based Biopharmaceuticals In Escherichia Coli

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