Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen

Antibodies are very important for therapeutic and diagnostic applications for infectious diseases. Immune libraries are useful for infectious disease applications because it would allow for better and selective enrichment of antibodies against disease antigens. The generations of antibody libraries...

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Main Author:	Hamidon, Nurul Hamizah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/45474/1/NURUL%20HAMIZAH%20HAMIDON.pdf
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id	my-usm-ep.45474
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spelling	my-usm-ep.454742019-09-18T07:28:14Z Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen 2017-05 Hamidon, Nurul Hamizah R735-854 Medical education. Medical schools. Research Antibodies are very important for therapeutic and diagnostic applications for infectious diseases. Immune libraries are useful for infectious disease applications because it would allow for better and selective enrichment of antibodies against disease antigens. The generations of antibody libraries are necessary to produce the monoclonal antibodies. Immune scFv antibody library was generated from six donors (tuberculosis patients). Conventional PCR method was used to amplify all possible antibody genes, which were then cloned into the pLABEL, phagemid vector. A two-step cloning procedure was done to generate the antibody library. The library size of the mini HC library and the scFv library constructed was approximately around 107 and 109 individual clones respectively. 2017-05 Thesis http://eprints.usm.my/45474/ http://eprints.usm.my/45474/1/NURUL%20HAMIZAH%20HAMIDON.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Hamidon, Nurul Hamizah Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen
description	Antibodies are very important for therapeutic and diagnostic applications for infectious diseases. Immune libraries are useful for infectious disease applications because it would allow for better and selective enrichment of antibodies against disease antigens. The generations of antibody libraries are necessary to produce the monoclonal antibodies. Immune scFv antibody library was generated from six donors (tuberculosis patients). Conventional PCR method was used to amplify all possible antibody genes, which were then cloned into the pLABEL, phagemid vector. A two-step cloning procedure was done to generate the antibody library. The library size of the mini HC library and the scFv library constructed was approximately around 107 and 109 individual clones respectively.
format	Thesis
qualification_level	Master's degree
author	Hamidon, Nurul Hamizah
author_facet	Hamidon, Nurul Hamizah
author_sort	Hamidon, Nurul Hamizah
title	Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen
title_short	Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen
title_full	Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen
title_fullStr	Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen
title_full_unstemmed	Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen
title_sort	development of an immune tuberculosis antibody library: monoclonal antibody generation for a-crystalline antigen
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2017
url	http://eprints.usm.my/45474/1/NURUL%20HAMIZAH%20HAMIDON.pdf
_version_	1747821517903757312

Development Of An Immune Tuberculosis Antibody Library: Monoclonal Antibody Generation For a-Crystalline Antigen

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